P0AFY8

PR
Gene name
seqA
Protein name
Negative modulator of initiation of replication
Names
Species
Escherichia coli (strain K12)
KEGG Pathway
eco:b0687
EC number
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

5-90 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

6 structures for P0AFY8

Entry ID Method Resolution Chain Position Source
1IU3 X-ray 300 A C/F 71-181 PDB
1J3E X-ray 250 A A 71-181 PDB
1LRR X-ray 265 A A/D 51-181 PDB
1XRX X-ray 215 A A/B/C/D 1-50 PDB
3FMT X-ray 298 A A/B/E/F 1-181 PDB
AF-P0AFY8-F1 Predicted AlphaFoldDB

No variants for P0AFY8

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P0AFY8

No associated diseases with P0AFY8

2 regional properties for P0AFY8

Type Name Position InterPro Accession
domain Replication modulator SeqA, C-terminal DNA-binding domain 71 - 179 IPR026577
domain Negative modulator of initiation of replication SeqA, N-terminal 1 - 36 IPR033761

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm
  • Localization is cell cycle-dependent
  • Localizes at midcell in newborn cells, then migrates in opposite directions toward cell quarter sites and remains tethered there until the cell divides
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
protein-containing complex A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together.
SeqA-DNA complex A protein-DNA complex that contains an oligomer of SeqA bound to GATC sites in methylated or newly-synthesized, hemi-methylated double-stranded DNA, with preference for the latter. Binding of SeqA to hemimethylated DNA sequesters oriC, prevents re-methylation of DNA by Dam and in turn stops premature re-initiation of replication during one replication cycle.

4 GO annotations of molecular function

Name Definition
DNA replication origin binding Binding to a DNA replication origin, a unique DNA sequence of a replicon at which DNA replication is initiated and proceeds bidirectionally or unidirectionally.
double-stranded methylated DNA binding Binding to double-stranded methylated DNA. Methylation of cytosine or adenine in DNA is an important mechanism for establishing stable heritable epigenetic marks.
hemi-methylated DNA-binding Binding to double-stranded hemi-methylated DNA at replication foci (one strand methylated, while the other strand is unmethylated). Methylation of cytosine or adenine in DNA is an important mechanism for establishing stable heritable epigenetic marks.
identical protein binding Binding to an identical protein or proteins.

3 GO annotations of biological process

Name Definition
negative regulation of DNA-templated DNA replication initiation Any process that stops, prevents, or reduces the frequency, rate or extent of initiation of DNA-dependent DNA replication.
regulation of DNA-templated transcription Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription.
response to radiation Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an electromagnetic radiation stimulus. Electromagnetic radiation is a propagating wave in space with electric and magnetic components. These components oscillate at right angles to each other and to the direction of propagation.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MKTIEVDDEL YSYIASHTKH IGESASDILR RMLKFSAASQ PAAPVTKEVR VASPAIVEAK
70 80 90 100 110 120
PVKTIKDKVR AMRELLLSDE YAEQKRAVNR FMLLLSTLYS LDAQAFAEAT ESLHGRTRVY
130 140 150 160 170 180
FAADEQTLLK NGNQTKPKHV PGTPYWVITN TNTGRKCSMI EHIMQSMQFP AELIEKVCGT
I