P0AEZ1

PR
Gene name
metF (b3941, JW3913)
Protein name
5,10-methylenetetrahydrofolate reductase
Names
Species
Escherichia coli (strain K12)
KEGG Pathway
eco:b3941
EC number
1.5.1.54: With NAD(+) or NADP(+) as acceptor
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-106 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

11 structures for P0AEZ1

Entry ID Method Resolution Chain Position Source
1B5T X-ray 250 A A/B/C 21-294 PDB
1ZP3 X-ray 185 A A/B/C 1-296 PDB
1ZP4 X-ray 185 A A/B/C 1-296 PDB
1ZPT X-ray 195 A A/B/C 1-296 PDB
1ZRQ X-ray 220 A A/B/C 1-296 PDB
2FMN X-ray 205 A A/B/C 1-296 PDB
2FMO X-ray 225 A A/B/C 1-296 PDB
3FST X-ray 165 A A/C/E 1-296 PDB
3FSU X-ray 170 A A/C/E 1-296 PDB
6PEY X-ray 288 A A/B/C 1-296 PDB
AF-P0AEZ1-F1 Predicted AlphaFoldDB

No variants for P0AEZ1

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P0AEZ1

No associated diseases with P0AEZ1

No regional properties for P0AEZ1

Type Name Position InterPro Accession
No domain, repeats, and functional sites for P0AEZ1

Functions

Description
EC Number 1.5.1.54 With NAD(+) or NADP(+) as acceptor
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
protein-containing complex A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together.

4 GO annotations of molecular function

Name Definition
FAD binding Binding to the oxidized form, FAD, of flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes.
methylenetetrahydrofolate reductase (NAD(P)H) activity Catalysis of the reaction: 5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H + H+.
methylenetetrahydrofolate reductase NADH activity Catalysis of the reaction: 5-methyltetrahydrofolate + NAD + = 5,10-methylenetetrahydrofolate + NADH + H+.
methylenetetrahydrofolate reductase NADPH activity Catalysis of the reaction: 5-methyltetrahydrofolate + NADP + = 5,10-methylenetetrahydrofolate + NADPH + H+.

2 GO annotations of biological process

Name Definition
methionine biosynthetic process The chemical reactions and pathways resulting in the formation of methionine (2-amino-4-(methylthio)butanoic acid), a sulfur-containing, essential amino acid found in peptide linkage in proteins.
tetrahydrofolate interconversion The chemical reactions and pathways by which one-carbon (C1) units are transferred between tetrahydrofolate molecules, to synthesise other tetrahydrofolate molecules.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MSFFHASQRD ALNQSLAEVQ GQINVSFEFF PPRTSEMEQT LWNSIDRLSS LKPKFVSVTY
70 80 90 100 110 120
GANSGERDRT HSIIKGIKDR TGLEAAPHLT CIDATPDELR TIARDYWNNG IRHIVALRGD
130 140 150 160 170 180
LPPGSGKPEM YASDLVTLLK EVADFDISVA AYPEVHPEAK SAQADLLNLK RKVDAGANRA
190 200 210 220 230 240
ITQFFFDVES YLRFRDRCVS AGIDVEIIPG ILPVSNFKQA KKFADMTNVR IPAWMAQMFD
250 260 270 280 290
GLDDDAETRK LVGANIAMDM VKILSREGVK DFHFYTLNRA EMSYAICHTL GVRPGL