P0AEB2

PR
Gene name
dacA (pfv, b0632, JW0627)
Protein name
D-alanyl-D-alanine carboxypeptidase DacA
Names
DD-carboxypeptidase, DD-peptidase, Beta-lactamase, Penicillin-binding protein 5, PBP-5
Species
Escherichia coli (strain K12)
KEGG Pathway
eco:b0632
EC number
3.4.16.4: Serine-type carboxypeptidases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

150-221 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

14 structures for P0AEB2

Entry ID Method Resolution Chain Position Source
1HD8 X-ray 230 A A 30-386 PDB
1NJ4 X-ray 190 A A 30-392 PDB
1NZO X-ray 185 A A 30-392 PDB
1NZU X-ray 200 A A 30-392 PDB
1SDN X-ray 250 A A 30-392 PDB
1Z6F X-ray 160 A A 30-392 PDB
3BEB X-ray 200 A A 30-386 PDB
3BEC X-ray 160 A A 30-386 PDB
3MZD X-ray 190 A A 30-386 PDB
3MZE X-ray 210 A A 30-386 PDB
3MZF X-ray 150 A A 30-386 PDB
5J8X X-ray 253 A A 30-392 PDB
6NTZ X-ray 220 A A 1-403 PDB
AF-P0AEB2-F1 Predicted AlphaFoldDB

1 variants for P0AEB2

Variant ID(s) Position Change Description Diseaes Association Provenance
134 G>D mutant dacA11191; inactive but still binds penicillin. Blocked in the release of the bound penicilloyl moiety; the mutant also fails to catalyze the D-alanine carboxypeptidase reaction as the hydrolysis of the acyl-enzyme formed with substrate is also blocked and the acyl-enzyme accumulates [UniProt] No

No associated diseases with P0AEB2

No regional properties for P0AEB2

Type Name Position InterPro Accession
No domain, repeats, and functional sites for P0AEB2

Functions

Description
EC Number 3.4.16.4 Serine-type carboxypeptidases
Subcellular Localization
  • Cell inner membrane; Peripheral membrane protein; Cytoplasmic side
  • N-terminal lies in the periplasmic space
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
integral component of periplasmic side of plasma membrane The component of the plasma membrane consisting of the gene products that penetrate only the periplasmic side of the membrane.
integral component of plasma membrane The component of the plasma membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.
outer membrane-bounded periplasmic space The region between the inner (cytoplasmic or plasma) membrane and outer membrane of organisms with two membranes such as Gram negative bacteria. These periplasmic spaces are relatively thick and contain a thin peptidoglycan layer (PGL), also referred to as a thin cell wall.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

4 GO annotations of molecular function

Name Definition
beta-lactamase activity Catalysis of the reaction: a beta-lactam + H2O = a substituted beta-amino acid.
carboxypeptidase activity Catalysis of the hydrolysis of a single C-terminal amino acid residue from a polypeptide chain.
penicillin binding Binding to penicillin, an antibiotic that contains the condensed beta-lactamthiazolidine ring system.
serine-type D-Ala-D-Ala carboxypeptidase activity Catalysis of the reaction: (Ac)2-L-Lys-D-alanyl-D-alanine + H2O = (Ac)2-L-Lys-D-alanine + D-alanine.

5 GO annotations of biological process

Name Definition
cell wall organization A process that results in the assembly, arrangement of constituent parts, or disassembly of the cell wall, the rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis.
peptidoglycan biosynthetic process The chemical reactions and pathways resulting in the formation of peptidoglycans, any of a class of glycoconjugates found in bacterial cell walls.
peptidoglycan metabolic process The chemical reactions and pathways involving peptidoglycans, any of a class of glycoconjugates found only in bacterial cell walls and consisting of strands of glycosaminoglycan cross-linked by oligopeptides to form a huge and rigid network.
proteolysis The hydrolysis of proteins into smaller polypeptides and/or amino acids by cleavage of their peptide bonds.
regulation of cell shape Any process that modulates the surface configuration of a cell.

1 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q8WVT3 TRAPPC12 Trafficking protein particle complex subunit 12 Homo sapiens (Human) PR
10 20 30 40 50 60
MNTIFSARIM KRLALTTALC TAFISAAHAD DLNIKTMIPG VPQIDAESYI LIDYNSGKVL
70 80 90 100 110 120
AEQNADVRRD PASLTKMMTS YVIGQAMKAG KFKETDLVTI GNDAWATGNP VFKGSSLMFL
130 140 150 160 170 180
KPGMQVPVSQ LIRGINLQSG NDACVAMADF AAGSQDAFVG LMNSYVNALG LKNTHFQTVH
190 200 210 220 230 240
GLDADGQYSS ARDMALIGQA LIRDVPNEYS IYKEKEFTFN GIRQLNRNGL LWDNSLNVDG
250 260 270 280 290 300
IKTGHTDKAG YNLVASATEG QMRLISAVMG GRTFKGREAE SKKLLTWGFR FFETVNPLKV
310 320 330 340 350 360
GKEFASEPVW FGDSDRASLG VDKDVYLTIP RGRMKDLKAS YVLNSSELHA PLQKNQVVGT
370 380 390 400
INFQLDGKTI EQRPLVVLQE IPEGNFFGKI IDYIKLMFHH WFG