P09436
Gene name |
ILS1 (YBL076C, YBL0734) |
Protein name |
Isoleucine--tRNA ligase, cytoplasmic |
Names |
Isoleucyl-tRNA synthetase, IleRS |
Species |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
KEGG Pathway |
sce:YBL076C |
EC number |
6.1.1.5: Ligases forming aminoacyl-tRNA and related compounds |
Protein Class |
|
Descriptions
The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
2 structures for P09436
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 7D5C | X-ray | 190 A | A | 1-984 | PDB |
| AF-P09436-F1 | Predicted | AlphaFoldDB |
7 variants for P09436
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| s02-83867 | 131 | R>S | No | SGRP | |
| s02-83524 | 246 | I>V | No | SGRP | |
| s02-82437 | 608 | K>R | No | SGRP | |
| s02-82219 | 681 | Q>K | No | SGRP | |
| s02-81499 | 921 | V>I | No | SGRP | |
| s02-81421 | 947 | L>M | No | SGRP | |
| s02-81091 | 1057 | E>Q | No | SGRP |
No associated diseases with P09436
4 regional properties for P09436
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| conserved_site | Aminoacyl-tRNA synthetase, class I, conserved site | 47 - 58 | IPR001412 |
| domain | Aminoacyl-tRNA synthetase, class Ia | 17 - 640 | IPR002300 |
| domain | Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding | 693 - 851 | IPR013155 |
| domain | Isoleucyl tRNA synthetase type 2, anticodon-binding domain | 642 - 835 | IPR033709 |
Functions
| Description | ||
|---|---|---|
| EC Number | 6.1.1.5 | Ligases forming aminoacyl-tRNA and related compounds |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
1 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
4 GO annotations of molecular function
| Name | Definition |
|---|---|
| aminoacyl-tRNA editing activity | The hydrolysis of an incorrectly aminoacylated tRNA. |
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| isoleucine-tRNA ligase activity | Catalysis of the reaction: L-isoleucine + ATP + tRNA(Ile) = L-isoleucyl-tRNA(Ile) + AMP + diphosphate + 2 H(+). |
| tRNA binding | Binding to a transfer RNA. |
1 GO annotations of biological process
| Name | Definition |
|---|---|
| isoleucyl-tRNA aminoacylation | The process of coupling isoleucine to isoleucyl-tRNA, catalyzed by isoleucyl-tRNA synthetase. The isoleucyl-tRNA synthetase is a class-I synthetase. The activated amino acid is transferred to the 2'-OH group of a isoleucine-accetping tRNA. The 2'-O-aminoacyl-tRNA will ultimately migrate to the 3' position via transesterification. |
No homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| No homologous proteins | ||||
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSESNAHFSF | PKEEEKVLSL | WDEIDAFHTS | LELTKDKPEF | SFFDGPPFAT | GTPHYGHILA |
| 70 | 80 | 90 | 100 | 110 | 120 |
| STIKDIVPRY | ATMTGHHVER | RFGWDTHGVP | IEHIIDKKLG | ITGKDDVFKY | GLENYNNECR |
| 130 | 140 | 150 | 160 | 170 | 180 |
| SIVMTYASDW | RKTIGRLGRW | IDFDNDYKTM | YPSFMESTWW | AFKQLHEKGQ | VYRGFKVMPY |
| 190 | 200 | 210 | 220 | 230 | 240 |
| STGLTTPLSN | FEAQQNYKDV | NDPAVTIGFN | VIGQEKTQLV | AWTTTPWTLP | SNLSLCVNAD |
| 250 | 260 | 270 | 280 | 290 | 300 |
| FEYVKIYDET | RDRYFILLES | LIKTLYKKPK | NEKYKIVEKI | KGSDLVGLKY | EPLFPYFAEQ |
| 310 | 320 | 330 | 340 | 350 | 360 |
| FHETAFRVIS | DDYVTSDSGT | GIVHNAPAFG | EEDNAACLKN | GVISEDSVLP | NAIDDLGRFT |
| 370 | 380 | 390 | 400 | 410 | 420 |
| KDVPDFEGVY | VKDADKLIIK | YLTNTGNLLL | ASQIRHSYPF | CWRSDTPLLY | RSVPAWFVRV |
| 430 | 440 | 450 | 460 | 470 | 480 |
| KNIVPQMLDS | VMKSHWVPNT | IKEKRFANWI | ANARDWNVSR | NRYWGTPIPL | WVSDDFEEVV |
| 490 | 500 | 510 | 520 | 530 | 540 |
| CVGSIKELEE | LTGVRNITDL | HRDVIDKLTI | PSKQGKGDLK | RIEEVFDCWF | ESGSMPYASQ |
| 550 | 560 | 570 | 580 | 590 | 600 |
| HYPFENTEKF | DERVPANFIS | EGLDQTRGWF | YTLAVLGTHL | FGSVPYKNVI | VSGIVLAADG |
| 610 | 620 | 630 | 640 | 650 | 660 |
| RKMSKSLKNY | PDPSIVLNKY | GADALRLYLI | NSPVLKAESL | KFKEEGVKEV | VSKVLLPWWN |
| 670 | 680 | 690 | 700 | 710 | 720 |
| SFKFLDGQIA | LLKKMSNIDF | QYDDSVKSDN | VMDRWILASM | QSLVQFIHEE | MGQYKLYTVV |
| 730 | 740 | 750 | 760 | 770 | 780 |
| PKLLNFIDEL | TNWYIRFNRR | RLKGENGVED | CLKALNSLFD | ALFTFVRAMA | PFTPFLSESI |
| 790 | 800 | 810 | 820 | 830 | 840 |
| YLRLKEYIPE | AVLAKYGKDG | RSVHFLSYPV | VKKEYFDEAI | ETAVSRMQSV | IDLGRNIREK |
| 850 | 860 | 870 | 880 | 890 | 900 |
| KTISLKTPLK | TLVILHSDES | YLKDVEALKN | YIIEELNVRD | VVITSDEAKY | GVEYKAVADW |
| 910 | 920 | 930 | 940 | 950 | 960 |
| PVLGKKLKKD | AKKVKDALPS | VTSEQVREYL | ESGKLEVAGI | ELVKGDLNAI | RGLPESAVQA |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| GQETRTDQDV | LIIMDTNIYS | ELKSEGLARE | LVNRIQKLRK | KCGLEATDDV | LVEYELVKDT |
| 1030 | 1040 | 1050 | 1060 | 1070 | |
| IDFEAIVKEH | FDMLSKTCRS | DIAKYDGSKT | DPIGDEEQSI | NDTIFKLKVF | KL |