P09339

PR
Gene name
citB (BSU18000)
Protein name
Aconitate hydratase A
Names
ACN, Aconitase, Aconitate/2-methylaconitate hydratase, Iron-responsive protein-like, IRP-like, RNA-binding protein
Species
Bacillus subtilis (strain 168)
KEGG Pathway
bsu:BSU18000
EC number
4.2.1.3: Hydro-lyases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

609-693 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P09339

Entry ID Method Resolution Chain Position Source
AF-P09339-F1 Predicted AlphaFoldDB

No variants for P09339

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P09339

No associated diseases with P09339

5 regional properties for P09339

Type Name Position InterPro Accession
domain Aconitase A/isopropylmalate dehydratase small subunit, swivel domain 707 - 834 IPR000573
domain Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain 85 - 577 IPR001030
binding_site Aconitase family, 4Fe-4S cluster binding site 442 - 458 IPR018136-1
binding_site Aconitase family, 4Fe-4S cluster binding site 508 - 521 IPR018136-2
domain Aconitase A, swivel domain 683 - 853 IPR044137

Functions

Description
EC Number 4.2.1.3 Hydro-lyases
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

1 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.

8 GO annotations of molecular function

Name Definition
2-methylisocitrate dehydratase activity Catalysis of the reaction: (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = cis-2-methylaconitate + H(2)O.
4 iron, 4 sulfur cluster binding Binding to a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.
aconitate hydratase activity Catalysis of the reaction: citrate = isocitrate. The reaction occurs in two steps: (1) citrate = cis-aconitate + H2O, (2) cis-aconitate + H2O = isocitrate. This reaction is the interconversion of citrate and isocitrate via the labile, enzyme-bound intermediate cis-aconitate. Water is removed from one part of the citrate molecule and added back to a different atom to form isocitrate.
citrate dehydratase activity Catalysis of the reaction: citrate = cis-aconitate + H2O.
iron-responsive element binding Binding to an iron-responsive element, a regulatory sequence found in the 5'- and 3'-untranslated regions of mRNAs encoding many iron-binding proteins.
metal ion binding Binding to a metal ion.
mRNA 3'-UTR binding Binding to a 3' untranslated region of an mRNA molecule.
mRNA binding Binding to messenger RNA (mRNA), an intermediate molecule between DNA and protein. mRNA includes UTR and coding sequences, but does not contain introns.

4 GO annotations of biological process

Name Definition
citrate metabolic process The chemical reactions and pathways involving citrate, 2-hydroxy-1,2,3-propanetricarboyxlate. Citrate is widely distributed in nature and is an important intermediate in the TCA cycle and the glyoxylate cycle.
propionate metabolic process, methylcitrate cycle The chemical reactions and pathways involving propionate that occur in the methylcitrate cycle.
regulation of sporulation Any process that modulates the frequency, rate or extent of sporulation, the process whose specific outcome is the progression of a spore over time, from its initiation to the mature structure.
tricarboxylic acid cycle A nearly universal metabolic pathway in which the acetyl group of acetyl coenzyme A is effectively oxidized to two CO2 and four pairs of electrons are transferred to coenzymes. The acetyl group combines with oxaloacetate to form citrate, which undergoes successive transformations to isocitrate, 2-oxoglutarate, succinyl-CoA, succinate, fumarate, malate, and oxaloacetate again, thus completing the cycle. In eukaryotes the tricarboxylic acid is confined to the mitochondria. See also glyoxylate cycle.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MANEQKTAAK DVFQARKTFT TNGKTYHYYS LKALEDSGIG KVSKLPYSIK VLLESVLRQV
70 80 90 100 110 120
DGFVIKKEHV ENLAKWGTAE LKDIDVPFKP SRVILQDFTG VPAVVDLASL RKAMAAVGGD
130 140 150 160 170 180
PDKINPEIPV DLVIDHSVQV DKAGTEDALA VNMDLEFERN AERYKFLSWA KKAFNNYQAV
190 200 210 220 230 240
PPATGIVHQV NLEFLASVVH AIEEDGELVT YPDTLVGTDS HTTMINGIGV LGWGVGGIEA
250 260 270 280 290 300
EAGMLGQPSY FPVPEVIGAK LVGKLPNGTT ATDLALKVTQ VLREKGVVGK FVEFFGPGIA
310 320 330 340 350 360
ELPLADRATI ANMAPEYGAT CGFFPVDEEA LNYLRLTGRD PEHIDVVEAY CRSNGLFYTP
370 380 390 400 410 420
DAEDPQFTDV VEIDLSQIEA NLSGPKRPQD LIPLSAMQET FKKQLVSPAG NQGFGLNAEE
430 440 450 460 470 480
ENKEIKFKLL NGEETVMKTG AIAIAAITSC TNTSNPYVLI GAGLVAKKAV ELGLKVPNYV
490 500 510 520 530 540
KTSLAPGSKV VTGYLVNSGL LPYMKELGFN LVGYGCTTCI GNSGPLSPEI EEAVAKNDLL
550 560 570 580 590 600
ITSVLSGNRN FEGRIHPLVK GNYLASPPLV VAYALAGTVN INLKTDPIGV GKDGQNVYFN
610 620 630 640 650 660
DIWPSMDEIN ALVKQTVTPE LFRKEYETVF DDNKRWNEIE TTDEALYKWD NDSTYIQNPP
670 680 690 700 710 720
FFEEMSVEPG KVEPLKGLRV VGKFGDSVTT DHISPAGAIG KDTPAGKYLQ EKGVSPRDFN
730 740 750 760 770 780
SYGSRRGNHE VMMRGTFANI RIKNQIAPGT EGGFTTYWPT GEVTSIYDAC MKYKEDKTGL
790 800 810 820 830 840
VVLAGKDYGM GSSRDWAAKG TNLLGIRTVI AESFERIHRS NLVFMGVLPL QFKQGENADT
850 860 870 880 890 900
LGLTGKEVIE VDVDETVRPR DLVTVRAINE DGNVTTFEAV VRFDSEVEID YYRHGGILQM
VLREKMKQS