AiPD: Autoinhibited Protein Database

Gene name	ptsI
Protein name	Phosphoenolpyruvate-protein phosphotransferase
Names	Phosphotransferase system, enzyme I
Species	Escherichia coli (strain K12)
KEGG Pathway	eco:b2416
EC number	2.7.3.9: Phosphotransferases with a nitrogenous group as acceptor
Protein Class

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

74-144 (Predicted disordered autoinhibitory region) PR

74-144 (Predicted disordered autoinhibitory region)

cis-regPred

No accessory elements

Entry ID	Method	Resolution	Chain	Position	Source
1EZA	NMR	-	A	1-258	PDB
1EZB	NMR	-	A	1-258	PDB
1EZC	NMR	-	A	1-258	PDB
1EZD	NMR	-	A	1-258	PDB
1ZYM	X-ray	250 A	A/B	1-258	PDB
2EZA	NMR	-	A	1-258	PDB
2EZB	NMR	-	A	1-258	PDB
2EZC	NMR	-	A	1-258	PDB
2HWG	X-ray	270 A	A/B	1-575	PDB
2KX9	Other	-	A/B	1-573	PDB
2L5H	Other	-	A/B	1-573	PDB
2MP0	NMR	-	A	1-258	PDB
2N5T	Other	-	A/B	1-575	PDB
2XDF	Other	-	A/B	1-573	PDB
3EZA	NMR	-	A	1-249	PDB
3EZB	NMR	-	A	1-258	PDB
3EZE	NMR	-	A	1-258	PDB
6V9K	X-ray	190 A	A/B	261-575	PDB
6VU0	X-ray	350 A	A/B	261-575	PDB
AF-P08839-F1	Predicted				AlphaFoldDB

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
No variants for P08839

Type	Name	Position	InterPro Accession
domain	PEP-utilising enzyme, C-terminal	250 - 541	IPR000121
domain	Phosphotransferase system, enzyme I-like	2 - 566	IPR006318
domain	PEP-utilising enzyme, mobile domain	152 - 222	IPR008279
domain	Phosphotransferase system, enzyme I N-terminal	4 - 126	IPR008731
active_site	PEP-utilising enzyme, active site	184 - 195	IPR018274
conserved_site	PEP-utilising enzyme, conserved site	447 - 465	IPR023151

		Description
EC Number	2.7.3.9	Phosphotransferases with a nitrogenous group as acceptor
Subcellular Localization	Cytoplasm
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

Name	Definition
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.

Name	Definition
identical protein binding	Binding to an identical protein or proteins.
kinase activity	Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
metal ion binding	Binding to a metal ion.
phosphoenolpyruvate-protein phosphotransferase activity	Catalysis of the reaction: phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine.

Name	Definition
phosphoenolpyruvate-dependent sugar phosphotransferase system	The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
phosphorylation	The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
P77439	fryA	Multiphosphoryl transfer protein 1	Escherichia coli (strain K12)	PR