P07313

EV
Gene name
MYLK2
Protein name
Myosin light chain kinase 2, skeletal/cardiac muscle
Names
MLCK2
Species
Oryctolagus cuniculus (Rabbit)
KEGG Pathway
ocu:100009051
EC number
2.7.11.18: Protein-serine/threonine kinases
Protein Class
SERINE/THREONINE-PROTEIN KINASE (PTHR24347)

Descriptions

Myosin light chain kinases (MLCK) are members of the family of Ca2+-calmodulin-dependent protein kinases. The autoinhibitory pseudosubstrate sequence was identified by sequence similarity with the human skeletal muscle MLCK (Q9H1R3). The mutational changes of acidic residues in the catalytic core of the kinase domain, which may interact with the basic residues in the pseudosubstrate sequence, increased the catalytic activity of the kinase domain.

Autoinhibitory domains (AIDs)

549-577 (Autoinhibitory pseudosubstrate) EV

Target domain

285-540 (Protein kinase domain)

Relief mechanism

Partner binding

Assay

Mutagenesis experiment

Accessory elements

437-459 (Activation loop from InterPro)

Target domain

297-552 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

4 structures for P07313

Entry ID Method Resolution Chain Position Source
2BBM NMR - B 578-603 PDB
2BBN NMR - B 578-603 PDB
6HR1 X-ray 190 A A/B 580-599 PDB
AF-P07313-F1 Predicted AlphaFoldDB

No variants for P07313

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P07313

No associated diseases with P07313

4 regional properties for P07313

Type Name Position InterPro Accession
domain Protein kinase domain 297 - 552 IPR000719
active_site Serine/threonine-protein kinase, active site 414 - 426 IPR008271
binding_site Protein kinase, ATP binding site 303 - 330 IPR017441
domain Myosin light chain kinase 2, catalytic domain 292 - 552 IPR042717

Functions

Description
EC Number 2.7.11.18 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Colocalizes with phosphorylated myosin light chain (RLCP) at filaments of the myofibrils
PANTHER Family PTHR24347 SERINE/THREONINE-PROTEIN KINASE
PANTHER Subfamily PTHR24347:SF373 MYOSIN LIGHT CHAIN KINASE 2, SKELETAL_CARDIAC MUSCLE
PANTHER Protein Class non-receptor serine/threonine protein kinase
protein modifying enzyme
PANTHER Pathway Category Inflammation mediated by chemokine and cytokine signaling pathway
MLCK
Cytoskeletal regulation by Rho GTPase
MLCK

1 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
calmodulin binding Binding to calmodulin, a calcium-binding protein with many roles, both in the calcium-bound and calcium-free states.
calmodulin-dependent protein kinase activity Calmodulin-dependent catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; and ATP + a protein threonine = ADP + protein threonine phosphate.
myosin light chain kinase activity Catalysis of the reaction: ATP + myosin-light-chain = ADP + myosin-light-chain phosphate.

1 GO annotations of biological process

Name Definition
regulation of muscle filament sliding Any process that modulates the frequency, rate or extent of muscle filament sliding.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MATENGAVEL GIQSLSTDEA SKGAASEESL AAEKDPAPPD PEKGPGPSDT KQDPDPSTPK
70 80 90 100 110 120
KDANTPAPEK GDVVPAQPSA GGSQGPAGEG GQVEAPAEGS AGKPAALPQQ TATAEASEKK
130 140 150 160 170 180
PEAEKGPSGH QDPGEPTVGK KVAEGQAAAR RGSPAFLHSP SCPAIIASTE KLPAQKPLSE
190 200 210 220 230 240
ASELIFEGVP ATPGPTEPGP AKAEGGVDLL AESQKEAGEK APGQADQAKV QGDTSRGIEF
250 260 270 280 290 300
QAVPSERPRP EVGQALCLPA REEDCFQILD DCPPPPAPFP HRIVELRTGN VSSEFSMNSK
310 320 330 340 350 360
EALGGGKFGA VCTCTEKSTG LKLAAKVIKK QTPKDKEMVM LEIEVMNQLN HRNLIQLYAA
370 380 390 400 410 420
IETPHEIVLF MEYIEGGELF ERIVDEDYHL TEVDTMVFVR QICDGILFMH KMRVLHLDLK
430 440 450 460 470 480
PENILCVNTT GHLVKIIDFG LARRYNPNEK LKVNFGTPEF LSPEVVNYDQ ISDKTDMWSL
490 500 510 520 530 540
GVITYMLLSG LSPFLGDDDT ETLNNVLSGN WYFDEETFEA VSDEAKDFVS NLIVKEQGAR
550 560 570 580 590 600
MSAAQCLAHP WLNNLAEKAK RCNRRLKSQI LLKKYLMKRR WKKNFIAVSA ANRFKKISSS
GALMALGV