P00890

PR
Gene name
CIT1
Protein name
Citrate synthase, mitochondrial
Names
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YNR001C
EC number
2.3.3.1: Acyl groups converted into alkyl groups on transfer
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-86 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

2 structures for P00890

Entry ID Method Resolution Chain Position Source
8GQZ X-ray 142 A A/B 1-479 PDB
AF-P00890-F1 Predicted AlphaFoldDB

6 variants for P00890

Variant ID(s) Position Change Description Diseaes Association Provenance
s14-630993 24 M>T No SGRP
s14-630963 34 A>V No SGRP
s14-630901 55 A>T No SGRP
s14-630634 144 A>S No SGRP
s14-630292 258 E>K No SGRP
s14-630088 326 L>M No SGRP

No associated diseases with P00890

1 regional properties for P00890

Type Name Position InterPro Accession
active_site Citrate synthase active site 355 - 367 IPR019810

Functions

Description
EC Number 2.3.3.1 Acyl groups converted into alkyl groups on transfer
Subcellular Localization
  • Mitochondrion matrix
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
mitochondrial intermembrane space The region between the inner and outer lipid bilayers of the mitochondrial envelope.
mitochondrial matrix The gel-like material, with considerable fine structure, that lies in the matrix space, or lumen, of a mitochondrion. It contains the enzymes of the tricarboxylic acid cycle and, in some organisms, the enzymes concerned with fatty acid oxidation.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

2 GO annotations of molecular function

Name Definition
citrate (Si)-synthase activity Catalysis of the reaction: acetyl-CoA + H2O + oxaloacetate = citrate + CoA, where the acetyl group is added to the si-face of oxaloacetate; acetyl-CoA thus provides the two carbon atoms of the pro-S carboxymethyl group.
citrate synthase activity Catalysis of the reaction: acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

4 GO annotations of biological process

Name Definition
acetyl-CoA catabolic process The chemical reactions and pathways resulting in the breakdown of acetyl-CoA, a derivative of coenzyme A in which the sulfhydryl group is acetylated.
carbohydrate metabolic process The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y.
citrate metabolic process The chemical reactions and pathways involving citrate, 2-hydroxy-1,2,3-propanetricarboyxlate. Citrate is widely distributed in nature and is an important intermediate in the TCA cycle and the glyoxylate cycle.
tricarboxylic acid cycle A nearly universal metabolic pathway in which the acetyl group of acetyl coenzyme A is effectively oxidized to two CO2 and four pairs of electrons are transferred to coenzymes. The acetyl group combines with oxaloacetate to form citrate, which undergoes successive transformations to isocitrate, 2-oxoglutarate, succinyl-CoA, succinate, fumarate, malate, and oxaloacetate again, thus completing the cycle. In eukaryotes the tricarboxylic acid is confined to the mitochondria. See also glyoxylate cycle.

1 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P08679 CIT2 Citrate synthase, peroxisomal Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
10 20 30 40 50 60
MSAILSTTSK SFLSRGSTRQ CQNMQKALFA LLNARHYSSA SEQTLKERFA EIIPAKAEEI
70 80 90 100 110 120
KKFKKEHGKT VIGEVLLEQA YGGMRGIKGL VWEGSVLDPE EGIRFRGRTI PEIQRELPKA
130 140 150 160 170 180
EGSTEPLPEA LFWLLLTGEI PTDAQVKALS ADLAARSEIP EHVIQLLDSL PKDLHPMAQF
190 200 210 220 230 240
SIAVTALESE SKFAKAYAQG VSKKEYWSYT FEDSLDLLGK LPVIASKIYR NVFKDGKITS
250 260 270 280 290 300
TDPNADYGKN LAQLLGYENK DFIDLMRLYL TIHSDHEGGN VSAHTTHLVG SALSSPYLSL
310 320 330 340 350 360
AAGLNGLAGP LHGRANQEVL EWLFKLREEV KGDYSKETIE KYLWDTLNAG RVVPGYGHAV
370 380 390 400 410 420
LRKTDPRYTA QREFALKHFP DYELFKLVST IYEVAPGVLT KHGKTKNPWP NVDSHSGVLL
430 440 450 460 470
QYYGLTEASF YTVLFGVARA IGVLPQLIID RAVGAPIERP KSFSTEKYKE LVKKIESKN