O89042

PR
Gene name
Pola1 (Pola)
Protein name
DNA polymerase alpha catalytic subunit
Names
DNA polymerase alpha catalytic subunit p180
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:85241
EC number
2.7.7.7: Nucleotidyltransferases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

93-170 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for O89042

Entry ID Method Resolution Chain Position Source
AF-O89042-F1 Predicted AlphaFoldDB

No variants for O89042

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for O89042

No associated diseases with O89042

16 regional properties for O89042

Type Name Position InterPro Accession
repeat HAT (Half-A-TPR) repeat 289 - 321 IPR003107-1
repeat HAT (Half-A-TPR) repeat 323 - 355 IPR003107-2
repeat HAT (Half-A-TPR) repeat 384 - 416 IPR003107-3
repeat HAT (Half-A-TPR) repeat 417 - 446 IPR003107-4
repeat HAT (Half-A-TPR) repeat 447 - 476 IPR003107-5
repeat HAT (Half-A-TPR) repeat 554 - 586 IPR003107-6
repeat HAT (Half-A-TPR) repeat 588 - 620 IPR003107-7
repeat HAT (Half-A-TPR) repeat 622 - 654 IPR003107-8
repeat HAT (Half-A-TPR) repeat 656 - 687 IPR003107-9
repeat HAT (Half-A-TPR) repeat 689 - 721 IPR003107-10
repeat HAT (Half-A-TPR) repeat 723 - 755 IPR003107-11
repeat HAT (Half-A-TPR) repeat 757 - 789 IPR003107-12
repeat HAT (Half-A-TPR) repeat 791 - 823 IPR003107-13
domain PRP1 splicing factor, N-terminal 13 - 169 IPR010491
repeat Tetratricopeptide repeat 709 - 742 IPR019734-1
repeat Tetratricopeptide repeat 841 - 874 IPR019734-2

Functions

Description
EC Number 2.7.7.7 Nucleotidyltransferases
Subcellular Localization
  • Nucleus
  • Cytoplasm, cytosol
  • In the cytosol, colocalizes with RNA:DNA hybrids with a speckled pattern
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

7 GO annotations of cellular component

Name Definition
alpha DNA polymerase:primase complex A complex of four polypeptides, comprising large and small DNA polymerase alpha subunits and two primase subunits, which are capable of catalyzing the synthesis of an RNA primer on the lagging strand of replicating DNA and the subsequent synthesis of a smal stretch of DNA. The smaller of the two primase subunits alone can catalyze oligoribonucleotide synthesis.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
nuclear envelope The double lipid bilayer enclosing the nucleus and separating its contents from the rest of the cytoplasm; includes the intermembrane space, a gap of width 20-40 nm (also called the perinuclear space).
nuclear matrix The dense fibrillar network lying on the inner side of the nuclear membrane.
nucleolus A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

11 GO annotations of molecular function

Name Definition
chromatin binding Binding to chromatin, the network of fibers of DNA, protein, and sometimes RNA, that make up the chromosomes of the eukaryotic nucleus during interphase.
DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
DNA replication origin binding Binding to a DNA replication origin, a unique DNA sequence of a replicon at which DNA replication is initiated and proceeds bidirectionally or unidirectionally.
DNA-directed DNA polymerase activity Catalysis of the reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1); the synthesis of DNA from deoxyribonucleotide triphosphates in the presence of a DNA template and a 3'hydroxyl group.
double-stranded DNA binding Binding to double-stranded DNA.
nucleotide binding Binding to a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose.
protein kinase binding Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.
purine nucleotide binding Binding to a purine nucleotide, a compound consisting of a purine nucleoside esterified with (ortho)phosphate.
pyrimidine nucleotide binding Binding to a pyrimidine nucleotide, a pyrimidine nucleoside esterified with (ortho)phosphate.
single-stranded DNA binding Binding to single-stranded DNA.
zinc ion binding Binding to a zinc ion (Zn).

12 GO annotations of biological process

Name Definition
DNA biosynthetic process The biosynthetic process resulting in the formation of DNA.
DNA repair The process of restoring DNA after damage. Genomes are subject to damage by chemical and physical agents in the environment (e.g. UV and ionizing radiations, chemical mutagens, fungal and bacterial toxins, etc.) and by free radicals or alkylating agents endogenously generated in metabolism. DNA is also damaged because of errors during its replication. A variety of different DNA repair pathways have been reported that include direct reversal, base excision repair, nucleotide excision repair, photoreactivation, bypass, double-strand break repair pathway, and mismatch repair pathway.
DNA replication The cellular metabolic process in which a cell duplicates one or more molecules of DNA. DNA replication begins when specific sequences, known as origins of replication, are recognized and bound by initiation proteins, and ends when the original DNA molecule has been completely duplicated and the copies topologically separated. The unit of replication usually corresponds to the genome of the cell, an organelle, or a virus. The template for replication can either be an existing DNA molecule or RNA.
DNA replication initiation The process in which DNA-dependent DNA replication is started; this begins with the ATP dependent loading of an initiator complex onto the DNA, this is followed by DNA melting and helicase activity. In bacteria, the gene products that enable the helicase activity are loaded after the initial melting and in archaea and eukaryotes, the gene products that enable the helicase activity are inactive when they are loaded and subsequently activate.
DNA replication, synthesis of RNA primer The synthesis of a short RNA polymer, usually 4-15 nucleotides long, using one strand of unwound DNA as a template; the RNA then serves as a primer from which DNA polymerases extend synthesis.
DNA strand elongation involved in DNA replication The process in which an existing DNA strand is extended by activities including the addition of nucleotides to the 3' end of the strand, complementary to an existing template, as part of DNA replication.
DNA synthesis involved in DNA repair Synthesis of DNA that proceeds from the broken 3' single-strand DNA end and uses the homologous intact duplex as the template.
double-strand break repair via nonhomologous end joining The repair of a double-strand break in DNA in which the two broken ends are rejoined with little or no sequence complementarity. Information at the DNA ends may be lost due to the modification of broken DNA ends. This term covers instances of separate pathways, called classical (or canonical) and alternative nonhomologous end joining (C-NHEJ and A-NHEJ). These in turn may further branch into sub-pathways, but evidence is still unclear.
lagging strand elongation The process in which an existing DNA strand is extended in a net 3' to 5' direction by activities including the addition of nucleotides to the 3' end of the strand, complementary to an existing template, as part of DNA replication. Lagging strand DNA elongation proceeds by discontinuous synthesis of short stretches of DNA, known as Okazaki fragments, from RNA primers; these fragments are then joined by DNA ligase. Although each segment of nascent DNA is synthesized in the 5' to 3' direction, the overall direction of lagging strand synthesis is 3' to 5', mirroring the progress of the replication fork.
leading strand elongation The process in which an existing DNA strand is extended continuously in a 5' to 3' direction by activities including the addition of nucleotides to the 3' end of the strand, complementary to an existing template, as part of DNA replication. Leading strand elongation proceeds in the same direction as the replication fork.
mitotic DNA replication initiation Any DNA replication initiation involved in mitotic cell cycle DNA replication.
regulation of type I interferon production Any process that modulates the frequency, rate, or extent of interferon type I production. Type I interferons include the interferon-alpha, beta, delta, episilon, zeta, kappa, tau, and omega gene families.

2 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P09884 POLA1 DNA polymerase alpha catalytic subunit Homo sapiens (Human) PR
P33609 Pola1 DNA polymerase alpha catalytic subunit Mus musculus (Mouse) PR
10 20 30 40 50 60
MAPVHGDDCK LETSAVSDSG SFVASRARRE KKSKKGRQEA LERLKKAKAG EKYKYEVEDL
70 80 90 100 110 120
TSVYEEVDEE QYSKLVQARQ DDDWIVDDDG IGYVEDGREI FDDDLEDDAL DTCGEGSDGK
130 140 150 160 170 180
AHRKDRKDVK KPSVTKPNNI KAMFIASAGK KTTDKTVDLS KDDLLGDILQ DLNTETPQIA
190 200 210 220 230 240
PPPVLIPKKK RSTGASPNPF SVHTATAVPS GKIASPVSRK EPPLTPVPLK RAEFAGDLAQ
250 260 270 280 290 300
PECPEDEQES GVIEFEDGDF DEPMDTEEVD EEEPVTAKIW DQESEPVEGV KHEADPETGT
310 320 330 340 350 360
TSFLDSFLPD VSCWDIDQKD ENSFLLQEVQ VDSNHLPLVK GADDEQVFQF YWLDAYEDPY
370 380 390 400 410 420
NQPGVVFLFG KVWVESAKTH VSCCVMVKNI ERTLYFLPRE MKIDLNTGKE TATPITMKDV
430 440 450 460 470 480
YEEFDSKISA KYKIMKFKSK IVEKNYAFEI PDVPEKSEYL EVRYSAEVPQ LPQNLKGETF
490 500 510 520 530 540
SHVFGTNTSS LELFLMNRKI KGPCWLEVKN PQLLNQPISW CKFEAMALKP DLVNVIKDVS
550 560 570 580 590 600
PPPLVVMSFS MKTMQNVQNH QHEIIAMAAL VHHNFPLDKA PPKPPFQTHF CVVSKPKDCI
610 620 630 640 650 660
FPCAFKEVIK KKNMEVEVAA TERTLLGFFL AKVHKLDPDI LVGHNICGFE LEVLLQRINE
670 680 690 700 710 720
CKVPFWSKIG RLRRSNMPKL GSRSGFGERN ATCGRMICDV EISVKELIHC KSYHLSELVQ
730 740 750 760 770 780
QILKTERIVI PTENIRNMYS EPSHLLYLLE HIWKDARFIL QIMCELNVLP LALQITNIAG
790 800 810 820 830 840
NIMSRTLMGG RSERNEFLLL HAFYENNYIV PDKQIFRKPQ QKPGDEDEEI DGDTNKYKKG
850 860 870 880 890 900
RKKAAYAGGL VLDPKVGFYD KFILLLDFNS LYPSIIQEFN ICFTTVQRVA SETLKATEDE
910 920 930 940 950 960
EQEQIPELPD PNLDMGILPR EIRKLVERRK QVKQLMKQQD LNPDLVLQYD IRQKALKLTA
970 980 990 1000 1010 1020
NSMYGCLGFS YSRFYAKPLA ALVTYKGREI LMHTKEMVQK MNLEVIYGDT DSIMINTNST
1030 1040 1050 1060 1070 1080
NLEEVFKLGN KVKNEVNKLY KLLEIDIDGV FKSLLLLKKK KYAALVVEPT SDGNYITKQE
1090 1100 1110 1120 1130 1140
LKGLDIVRRD WCDLAKDTGN FVIGQILSDQ SRDTIVENIQ KRLIEIGENV LNGSVPVSQF
1150 1160 1170 1180 1190 1200
EINKALTKDP QDYPDKKSLP HVHVALWINS QGGRKVKAGD TVSYVICQDG SNLPATQRAY
1210 1220 1230 1240 1250 1260
APEQLQKQDN LAIDTQYYLA QQIHPVVARI CEPIDGIDAV LIALWLGLDS TQFRVHQYHK
1270 1280 1290 1300 1310 1320
DEENDALLGG PAQLTDEEKY KDCEKFKCLC PSCGTENIYD NVFEGSGMDM EPSLNRCSNI
1330 1340 1350 1360 1370 1380
DCKASPATFM VQLSNKLIMD IRRCIKKYYD GWLICEEPTC RNRIRRLPLH FSRNGPLCPA
1390 1400 1410 1420 1430 1440
CMKAVLRPEY SDKSLYTQLC FYRYIFDADC ALEKLPEHEK DKLKKQFFTP RVLQDYRKVK
1450
NIAEHFLSWS G