O88508
SS
Gene name |
Dnmt3a |
Protein name |
DNA |
Names |
cytosine-5-methyltransferase 3A , Dnmt3a , EC 2.1.1.37 , Cysteine methyltransferase DNMT3A , EC 2.1.1.- , DNA methyltransferase MmuIIIA , DNA MTase MmuIIIA , M.MmuIIIA |
Species |
Rattus norvegicus (Rat) |
KEGG Pathway |
mmu:13435 |
EC number |
|
Protein Class |
|
Descriptions
EB1 including a CH domain and the C-terminal dimerization domain is a plus-end tracking protein that binds to the plus-end of microtubules (MT) and regulates the dynamics of the microtubule cytoskeleton. The CH domain is responsible for MT-binding and inhibited by the binding of the CH domain and the central region of the C-terminal domain at the FYF motif. SxIP competes with the CH domain and binds on the FYF motif of the C-terminal domain, and activates the CH domain.
Autoinhibitory domains (AIDs)
Target domain |
630-908 (MTase domain) |
Relief mechanism |
Partner binding |
Assay |
Structural analysis, Deletion assay |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
3 structures for O88508
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 3SW9 | X-ray | 305 A | P/Q | 39-50 | PDB |
| 3SWC | X-ray | 233 A | P/Q | 39-50 | PDB |
| AF-O88508-F1 | Predicted | AlphaFoldDB |
34 variants for O88508
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs3389231754 | 9 | T>N | No | EVA | |
| rs3389198618 | 40 | A>T | No | EVA | |
| rs3389224899 | 90 | L>* | No | EVA | |
| rs3389198637 | 90 | L>F | No | EVA | |
| rs3389231745 | 96 | P>T | No | EVA | |
| rs3389230012 | 121 | P>L | No | EVA | |
| rs3389240139 | 123 | E>K | No | EVA | |
| rs3389227044 | 143 | A>T | No | EVA | |
| rs49742619 | 151 | Q>P | No | EVA | |
| rs3389240146 | 164 | G>S | No | EVA | |
| rs3389243978 | 165 | R>P | No | EVA | |
| rs3389227060 | 186 | F>L | No | EVA | |
| rs3389198604 | 223 | V>M | No | EVA | |
| rs3389227075 | 247 | T>K | No | EVA | |
| rs3389230065 | 311 | M>I | No | EVA | |
| rs3389161396 | 314 | R>Q | No | EVA | |
| rs3389190604 | 317 | A>T | No | EVA | |
| rs3389202728 | 372 | A>S | No | EVA | |
| rs3389240057 | 380 | F>I | No | EVA | |
| rs3401631014 | 422 | E>EGKCGFPSPCCTGPDHIYLLVAPGP*DPRAGSH | No | EVA | |
| rs3389224836 | 422 | E>G | No | EVA | |
| rs3389237260 | 423 | E>K | No | EVA | |
| rs3389230099 | 470 | R>C | No | EVA | |
| rs3389202784 | 476 | V>M | No | EVA | |
| rs3389226841 | 545 | C>G | No | EVA | |
| rs3389221182 | 554 | F>L | No | EVA | |
| rs3403183054 | 557 | E>D | No | EVA | |
| rs3389202780 | 602 | Q>* | No | EVA | |
| rs3389198564 | 610 | D>V | No | EVA | |
| rs3389231688 | 719 | L>H | No | EVA | |
| rs3389227022 | 827 | R>K | No | EVA | |
| rs3389229060 | 856 | W>* | No | EVA | |
| rs3389216010 | 872 | D>H | No | EVA | |
| rs3389190521 | 886 | G>S | No | EVA |
No associated diseases with O88508
15 regional properties for O88508
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Protein kinase domain | 466 - 756 | IPR000719 |
| domain | Serine-threonine/tyrosine-protein kinase, catalytic domain | 466 - 743 | IPR001245 |
| domain | Immunoglobulin subtype 2 | 50 - 114 | IPR003598-1 |
| domain | Immunoglobulin subtype 2 | 161 - 229 | IPR003598-2 |
| domain | Immunoglobulin subtype 2 | 260 - 340 | IPR003598-3 |
| domain | Immunoglobulin subtype | 44 - 124 | IPR003599-1 |
| domain | Immunoglobulin subtype | 155 - 240 | IPR003599-2 |
| domain | Immunoglobulin subtype | 254 - 351 | IPR003599-3 |
| domain | Immunoglobulin-like domain | 39 - 108 | IPR007110-1 |
| domain | Immunoglobulin-like domain | 145 - 238 | IPR007110-2 |
| domain | Immunoglobulin-like domain | 247 - 349 | IPR007110-3 |
| active_site | Tyrosine-protein kinase, active site | 607 - 619 | IPR008266 |
| domain | Immunoglobulin I-set | 160 - 239 | IPR013098 |
| binding_site | Protein kinase, ATP binding site | 472 - 502 | IPR017441 |
| domain | Tyrosine-protein kinase, catalytic domain | 466 - 743 | IPR020635 |
Functions
9 GO annotations of cellular component
| Name | Definition |
|---|---|
| catalytic complex | A protein complex which is capable of catalytic activity. |
| chromosome, centromeric region | The region of a chromosome that includes the centromeric DNA and associated proteins. In monocentric chromosomes, this region corresponds to a single area of the chromosome, whereas in holocentric chromosomes, it is evenly distributed along the chromosome. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| euchromatin | A dispersed and relatively uncompacted form of chromatin that is in a transcription-competent conformation. |
| heterochromatin | A compact and highly condensed form of chromatin that is refractory to transcription. |
| nuclear matrix | The dense fibrillar network lying on the inner side of the nuclear membrane. |
| nucleoplasm | That part of the nuclear content other than the chromosomes or the nucleolus. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| XY body | A structure found in a male mammalian spermatocyte containing an unpaired X chromosome that has become densely heterochromatic, silenced and localized at the nuclear periphery. |
10 GO annotations of molecular function
| Name | Definition |
|---|---|
| chromatin binding | Binding to chromatin, the network of fibers of DNA, protein, and sometimes RNA, that make up the chromosomes of the eukaryotic nucleus during interphase. |
| DNA (cytosine-5-)-methyltransferase activity | Catalysis of the reaction |
| DNA (cytosine-5-)-methyltransferase activity, acting on CpN substrates | Catalysis of the reaction |
| DNA binding | Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). |
| identical protein binding | Binding to an identical protein or proteins. |
| metal ion binding | Binding to a metal ion. |
| protein-cysteine methyltransferase activity | Catalysis of the reaction |
| RNA polymerase II cis-regulatory region sequence-specific DNA binding | Binding to a specific upstream regulatory DNA sequence (transcription factor recognition sequence or binding site) located in cis relative to the transcription start site (i.e., on the same strand of DNA) of a gene transcribed by RNA polymerase II. |
| RNA polymerase II-specific DNA-binding transcription factor binding | Binding to a sequence-specific DNA binding RNA polymerase II transcription factor, any of the factors that interact selectively and non-covalently with a specific DNA sequence in order to modulate transcription. |
| unmethylated CpG binding | Binding to uan nmethylated CpG motif. Unmethylated CpG dinucleotides are often associated with gene promoters. |
24 GO annotations of biological process
| Name | Definition |
|---|---|
| C-5 methylation of cytosine | The covalent transfer of a methyl group to C-5 of cytosine in a DNA molecule. |
| cellular response to amino acid stimulus | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an amino acid stimulus. An amino acid is a carboxylic acids containing one or more amino groups. |
| cellular response to bisphenol A | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a bisphenol A stimulus. |
| cellular response to ethanol | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an ethanol stimulus. |
| cellular response to hypoxia | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating lowered oxygen tension. Hypoxia, defined as a decline in O2 levels below normoxic levels of 20.8 - 20.95%, results in metabolic adaptation at both the cellular and organismal level. |
| DNA methylation | The covalent transfer of a methyl group to either N-6 of adenine or C-5 or N-4 of cytosine. |
| DNA methylation on cytosine | The covalent transfer of a methyl group to C-5 or N-4 of cytosine in a DNA molecule. |
| DNA methylation-dependent heterochromatin formation | Repression of transcription by methylation of DNA, leading to the formation of heterochromatin. |
| genomic imprinting | The establishment of epigenetic modifications (imprints) during gametogenesis, leading to an asymmetry between the maternal and paternal alleles, leading to differential expression of the corresponding alleles. This can happen through heterochromatin formation or through differential chromatin loop formation. |
| hepatocyte apoptotic process | Any apoptotic process in a hepatocyte, the main structural component of the liver. |
| negative regulation of DNA-templated transcription | Any process that stops, prevents, or reduces the frequency, rate or extent of cellular DNA-templated transcription. |
| negative regulation of gene expression via CpG island methylation | An epigenetic gene regulation mechanism that negatively regulates gene expression by methylation of cytosine residues in chromosomal CpG islands. CpG islands are genomic regions that contain a high frequency of the CG dinucleotide and are often associated with the transcription start site of genes. |
| negative regulation of transcription by RNA polymerase II | Any process that stops, prevents, or reduces the frequency, rate or extent of transcription mediated by RNA polymerase II. |
| neuron differentiation | The process in which a relatively unspecialized cell acquires specialized features of a neuron. |
| positive regulation of cellular response to hypoxia | Any process that activates or increases the frequency, rate or extent of cellular response to hypoxia. |
| post-fertilization epigenetic regulation of gene expression | The covalent transfer of a methyl group to C-5 of cytosine that contributes to the epigenetic regulation of embryonic gene expression. |
| response to cocaine | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cocaine stimulus. Cocaine is a crystalline alkaloid obtained from the leaves of the coca plant. |
| response to estradiol | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of stimulus by estradiol, a C18 steroid hormone hydroxylated at C3 and C17 that acts as a potent estrogen. |
| response to ionizing radiation | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a ionizing radiation stimulus. Ionizing radiation is radiation with sufficient energy to remove electrons from atoms and may arise from spontaneous decay of unstable isotopes, resulting in alpha and beta particles and gamma rays. Ionizing radiation also includes X-rays. |
| response to lead ion | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a lead ion stimulus. |
| response to toxic substance | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a toxic stimulus. |
| response to vitamin A | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a vitamin A stimulus. |
| response to xenobiotic stimulus | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a xenobiotic, a compound foreign to the organim exposed to it. It may be synthesized by another organism (like ampicilin) or it can be a synthetic chemical. |
| spermatogenesis | The developmental process by which male germ line stem cells self renew or give rise to successive cell types resulting in the development of a spermatozoa. |
5 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q4W5Z4 | DNMT3A | DNA | Gallus gallus (Chicken) | SS |
| Q9UBC3 | DNMT3B | DNA | Homo sapiens (Human) | EV |
| Q9Y6K1 | DNMT3A | DNA (cytosine-5)-methyltransferase 3A | Homo sapiens (Human) | EV |
| O88509 | Dnmt3b | DNA | Mus musculus (Mouse) | SS |
| Q1LZ53 | Dnmt3a | DNA | Rattus norvegicus (Rat) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MAVNVYSTSV | TSDNLSRHDM | LAWINESLQL | NLTKIEQLCS | GAAYCQFMDM | LFPGSIALKK |
| 70 | 80 | 90 | 100 | 110 | 120 |
| VKFQAKLEHE | YIQNFKILQA | GFKRMGVDKI | IPVDKLVKGK | FQDNFEFVQW | FKKFFDANYD |
| 130 | 140 | 150 | 160 | 170 | 180 |
| GKEYDPVAAR | QGQETAVAPS | LVAPALSKPK | KPLGSGSAAP | QRPIATQRTT | AAPKAGPGMV |
| 190 | 200 | 210 | 220 | 230 | 240 |
| RKNPGMGNGD | DEAAELMQQV | KVLKLTVEDL | EKERDFYFGK | LRNIELICQE | NEGENDPVLQ |
| 250 | 260 | ||||
| RIVDILYATD | EGFVIPDEGG | PQEEQEEY |