O74833
PR
Gene name |
lig4 (SPCC1183.05c) |
Protein name |
DNA ligase 4 |
Names |
DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4 |
Species |
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
KEGG Pathway |
spo:SPCC1183.05c |
EC number |
6.5.1.1: Forming phosphoric ester bonds |
Protein Class |
|
Descriptions
The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for O74833
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-O74833-F1 | Predicted | AlphaFoldDB |
No variants for O74833
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for O74833 | |||||
No associated diseases with O74833
7 regional properties for O74833
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | BRCT domain | 660 - 753 | IPR001357-1 |
| domain | BRCT domain | 853 - 902 | IPR001357-2 |
| domain | DNA ligase, ATP-dependent, N-terminal | 25 - 212 | IPR012308 |
| domain | DNA ligase, ATP-dependent, C-terminal | 488 - 603 | IPR012309 |
| domain | DNA ligase, ATP-dependent, central | 254 - 500 | IPR012310 |
| conserved_site | DNA ligase, ATP-dependent, conserved site | 279 - 287 | IPR016059 |
| domain | DNA Ligase 4, adenylation domain | 242 - 467 | IPR044125 |
Functions
| Description | ||
|---|---|---|
| EC Number | 6.5.1.1 | Forming phosphoric ester bonds |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
4 GO annotations of cellular component
| Name | Definition |
|---|---|
| chromatin | The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome. |
| DNA ligase IV complex | A eukaryotically conserved protein complex that contains DNA ligase IV and is involved in DNA repair by non-homologous end joining; in addition to the ligase, the complex also contains XRCC4 or a homolog, e.g. Saccharomyces Lif1p. |
| nucleolus | A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
4 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| DNA binding | Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). |
| DNA ligase (ATP) activity | Catalysis of the reaction: ATP + deoxyribonucleotide(n) + deoxyribonucleotide(m) = AMP + diphosphate + deoxyribonucleotide(n+m). |
| metal ion binding | Binding to a metal ion. |
6 GO annotations of biological process
| Name | Definition |
|---|---|
| DNA biosynthetic process | The biosynthetic process resulting in the formation of DNA. |
| DNA ligation involved in DNA repair | The re-formation of a broken phosphodiester bond in the DNA backbone, carried out by DNA ligase, that contributes to DNA repair. |
| DNA recombination | Any process in which a new genotype is formed by reassortment of genes resulting in gene combinations different from those that were present in the parents. In eukaryotes genetic recombination can occur by chromosome assortment, intrachromosomal recombination, or nonreciprocal interchromosomal recombination. Interchromosomal recombination occurs by crossing over. In bacteria it may occur by genetic transformation, conjugation, transduction, or F-duction. |
| DNA replication | The cellular metabolic process in which a cell duplicates one or more molecules of DNA. DNA replication begins when specific sequences, known as origins of replication, are recognized and bound by initiation proteins, and ends when the original DNA molecule has been completely duplicated and the copies topologically separated. The unit of replication usually corresponds to the genome of the cell, an organelle, or a virus. The template for replication can either be an existing DNA molecule or RNA. |
| double-strand break repair via nonhomologous end joining | The repair of a double-strand break in DNA in which the two broken ends are rejoined with little or no sequence complementarity. Information at the DNA ends may be lost due to the modification of broken DNA ends. This term covers instances of separate pathways, called classical (or canonical) and alternative nonhomologous end joining (C-NHEJ and A-NHEJ). These in turn may further branch into sub-pathways, but evidence is still unclear. |
| nucleotide-excision repair, DNA gap filling | Repair of the gap in the DNA helix by DNA polymerase and DNA ligase after the portion of the strand containing the lesion has been removed by pyrimidine-dimer repair enzymes. |
No homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| No homologous proteins | ||||
| 10 | 20 | 30 | 40 | 50 | 60 |
| MDEAKETVFT | KPQNHSSTLE | FYDFVTTLLE | PLSRIGKTRK | SKTSNLDPYE | LKRKILLDYF |
| 70 | 80 | 90 | 100 | 110 | 120 |
| NKWRQHVGPD | LYPLLRLMLP | DLDRERGSYG | FKEFGLGKLF | IRAMHLSPTS | EDAKSLKNWR |
| 130 | 140 | 150 | 160 | 170 | 180 |
| GSESKHTGDF | STMLQDILQR | RAYRTFPGAF | TVGDVNALLD | QLADASSEDT | RVNILEQFYR |
| 190 | 200 | 210 | 220 | 230 | 240 |
| SLSPLELRWL | IPILLKVRKY | GTSEKFILSV | FHPDAARLYR | LCSSLKRICW | ELYDPSRSLD |
| 250 | 260 | 270 | 280 | 290 | 300 |
| ETETDVEVFS | CFQPQLANFK | KKDLHQTLEA | MGNKPFWIEE | KLDGERIQLH | MSSGKFQFYS |
| 310 | 320 | 330 | 340 | 350 | 360 |
| RNARSYTYAY | GSSYFDEQSR | LTQYIIGAFD | KRISQIILDG | EMVTWDPVLE | TVIPYGSLRS |
| 370 | 380 | 390 | 400 | 410 | 420 |
| IFEDSSSHSS | YSPYYVVFDI | LYLNGKSLVK | YSLESRRRIL | EKVIVRESHR | MSILPYKVGS |
| 430 | 440 | 450 | 460 | 470 | 480 |
| TIEDIEAELR | NVIQEGSEGL | VIKKPSGSYH | LGERMDDWIK | VKPYYLQGFG | EDLDCLILGG |
| 490 | 500 | 510 | 520 | 530 | 540 |
| YFGRGKQSGK | INSFLCGLRM | DYTPKDHSEK | FQSFVRVGGG | FTYFDRDIIR | KETEGKWLPW |
| 550 | 560 | 570 | 580 | 590 | 600 |
| SSDALEYMEL | AGTKQDFEKP | DMWIHPKDSL | VLQIKAAEVV | VSNRFKTNYT | LRFPRLEKVR |
| 610 | 620 | 630 | 640 | 650 | 660 |
| LDRSWKDALT | INEFFTLKNA | VEKQDNVSFH | VNKKRKVSQK | REKQKKFLYD | EPTFKKEASP |
| 670 | 680 | 690 | 700 | 710 | 720 |
| HSDVLKNLHF | VVLPPTELHE | TKAGLQQIII | ENGGLIHQGV | GNFGKERLFL | VADRVSTRVS |
| 730 | 740 | 750 | 760 | 770 | 780 |
| IERSKNMCTI | IRSQWVMDSV | NNQRLMPQWS | YLLFSKDEKY | SWKTALESLS | AKSLSNLLVE |
| 790 | 800 | 810 | 820 | 830 | 840 |
| LKQLDLSKEY | SKISDDTSIL | NLTISKEEAS | FVGAFPFLKF | TVFLDLKGIE | NSELYDVRMG |
| 850 | 860 | 870 | 880 | 890 | 900 |
| QYRLTKCILL | WNGATIEKDI | SSKKLTHVVM | FVEDSTRLEQ | LTKACELYQI | EPKFVNFEWV |
| 910 | |||||
| VNEWKKASTN | ILG |