O59245

PR
Gene name
rtcB (PH1602)
Protein name
tRNA-splicing ligase RtcB
Names
3'-phosphate/5'-hydroxy nucleic acid ligase, tRNA ligase RtcB
Species
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
KEGG Pathway
pho:PH1602
EC number
6.5.1.8: Forming phosphoric ester bonds
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

535-616 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

14 structures for O59245

Entry ID Method Resolution Chain Position Source
1UC2 X-ray 215 A PDB
4DWQ X-ray 225 A PDB
4DWR X-ray 148 A PDB
4ISJ X-ray 234 A PDB
4ISZ X-ray 230 A PDB
4IT0 X-ray 240 A PDB
7LFQ X-ray 270 A A 406-871 PDB
8DC9 X-ray 247 A A/B 406-871 PDB
8DCA X-ray 243 A A/B 406-871 PDB
8DCB X-ray 260 A A/B 406-871 PDB
8DCD X-ray 222 A A/B 406-871 PDB
8DCF X-ray 242 A A/B 406-871 PDB
8DCG X-ray 235 A A/B 406-871 PDB
AF-O59245-F1 Predicted AlphaFoldDB

No variants for O59245

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for O59245

No associated diseases with O59245

3 regional properties for O59245

Type Name Position InterPro Accession
domain Elp3/MiaA/NifB-like, radical SAM core domain 128 - 337 IPR006638
domain Radical SAM 71 - 364 IPR007197
domain Lipoyl synthase, N-terminal 74 - 112 IPR031691

Functions

Description
EC Number 6.5.1.8 Forming phosphoric ester bonds
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

No GO annotations of cellular component

Name Definition
No GO annotations for cellular component

6 GO annotations of molecular function

Name Definition
DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
endonuclease activity Catalysis of the hydrolysis of ester linkages within nucleic acids by creating internal breaks.
GMP binding Binding to GMP, guanosine monophosphate.
GTP binding Binding to GTP, guanosine triphosphate.
manganese ion binding Binding to a manganese ion (Mn).
RNA ligase (ATP) activity Catalysis of the reaction: ATP + ribonucleotide(n) + ribonucleotide(m) = AMP + diphosphate + ribonucleotide(n+m).

4 GO annotations of biological process

Name Definition
intein-mediated protein splicing The removal of an internal amino acid sequence (an intein) from a protein during protein maturation; the excision of inteins is precise and the N- and C-terminal exteins are joined by a normal peptide bond. Protein splicing involves 4 nucleophilic displacements by the 3 conserved splice junction residues.
intron homing Lateral transfer of an intron to a homologous allele that lacks the intron, mediated by a site-specific endonuclease encoded within the mobile intron.
RNA splicing, via endonucleolytic cleavage and ligation Splicing of RNA via recognition of the folded RNA structure that brings the 5' and 3' splice sites into proximity and cleavage of the RNA at both the 3' and 5' splice sites by an endonucleolytic mechanism, followed by ligation of the exons.
tRNA processing The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MVVPLKRIDK IRWEIPKFDK RMRVPGRVYA DEVLLEKMKN DRTLEQATNV AMLPGIYKYS
70 80 90 100 110 120
IVMPDGHQGY GFPIGGVAAF DVKEGVISPG GIGYDINCLA PGTRVLTEHG YWLKIEEMPE
130 140 150 160 170 180
KFKLQRLRVY NIEEGHNDFS KVVFVAEREV GSEEKAIRIV TESGKVIEGS EDHPVLTPEG
190 200 210 220 230 240
YVYLRNVKEG DYILVYPFEG VPYEEKKGVI LDESAFEGED PQVVKFLRER NLIPLQWKDP
250 260 270 280 290 300
KVGILARILG FALANGYISE NDNLTFHGKE EVLREVRKDL EELGIEAIVA EEDKLKVTSR
310 320 330 340 350 360
EFAFLLEKLG MAHDSIPEWI IEGPLWIKRN FLAGLFGANG SIVEFKGDVP LPITLTHSRE
370 380 390 400 410 420
LLNDVSRILE GFKVRAKIKM GKNGSYQLVI EDEDSIRNFL GRINYEYDPE KKARGLIAYA
430 440 450 460 470 480
YLKFKELMKG NLMTFEEFAR DRGYEGGFVA EKVIEVKSVK PEYDKFYDIG VYHSAHNFIA
490 500 510 520 530 540
NGIVVHNCGV RLIRTNLTEK EVRPRIKQLV DTLFKNVPSG VGSQGRIKLH WTQIDDVLVD
550 560 570 580 590 600
GAKWAVDNGY GWERDLERLE EGGRMEGADP EAVSQRAKQR GAPQLGSLGS GNHFLEVQVV
610 620 630 640 650 660
DKIFDPEVAK AYGLFEGQVV VMVHTGSRGL GHQVASDYLR IMERAIRKYR IPWPDRELVS
670 680 690 700 710 720
VPFQSEEGQR YFSAMKAAAN FAWANRQMIT HWVRESFQEV FKQDPEGDLG MDIVYDVAHN
730 740 750 760 770 780
IGKVEEHEVD GKRVKVIVHR KGATRAFPPG HEAVPRLYRD VGQPVLIPGS MGTASYILAG
790 800 810 820 830 840
TEGAMKETFG STCHGAGRVL SRKAATRQYR GDRIRQELLN RGIYVRAASM RVVAEEAPGA
850 860 870
YKNVDNVVKV VSEAGIAKLV ARMRPIGVAK G