O57921

PR
Gene name
nadE
Protein name
NH(3)-dependent NAD(+) synthetase
Names
Species
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
KEGG Pathway
pho:PH0182
EC number
6.3.1.5: Acid--ammonia (or amine) ligases (amide synthases)
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

212-257 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

2 structures for O57921

Entry ID Method Resolution Chain Position Source
2E18 X-ray 210 A A/B 1-257 PDB
AF-O57921-F1 Predicted AlphaFoldDB

No variants for O57921

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for O57921

No associated diseases with O57921

2 regional properties for O57921

Type Name Position InterPro Accession
conserved_site Small acid-soluble spore protein, alpha/beta-type, conserved site 214 - 223 IPR018126
domain NAD/GMP synthase 11 - 245 IPR022310

Functions

Description
EC Number 6.3.1.5 Acid--ammonia (or amine) ligases (amide synthases)
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

1 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

6 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
double-stranded DNA binding Binding to double-stranded DNA.
glutaminase activity Catalysis of the reaction: L-glutamine + H2O = L-glutamate + NH3.
metal ion binding Binding to a metal ion.
NAD+ synthase (glutamine-hydrolyzing) activity Catalysis of the reaction: ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate.
NAD+ synthase activity Catalysis of the reaction: ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.

2 GO annotations of biological process

Name Definition
DNA topological change The process in which a transformation is induced in the topological structure of a double-stranded DNA helix, resulting in a change in linking number.
NAD biosynthetic process The chemical reactions and pathways resulting in the formation of nicotinamide adenine dinucleotide, a coenzyme present in most living cells and derived from the B vitamin nicotinic acid; biosynthesis may be of either the oxidized form, NAD, or the reduced form, NADH.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MRILDYDKVI ERILEFIREK GNNGVVIGIS GGVDSATVAY LATKALGKEK VLGLIMPYFE
70 80 90 100 110 120
NKDVEDAKLV AEKLGIGYKV INIKPIVDSF VENLELNLDR KGLGNIMSRT RMIMLYAHAN
130 140 150 160 170 180
SLGRIVLGTS NRSEFLTGYF TKWGDGASDY APIINLYKTE VWEIAKRIGV PERIVKKKPS
190 200 210 220 230 240
AGLWEGQTDE DELGISYNLL DEILWRMIDL KIGKEEIAKD LGIPLSLVER VEELIKKSEH
250
KRRLPIGPSF EDLIVGP