O14098

PR
Gene name
lsk1
Protein name
CTD kinase subunit alpha
Names
CTDK-I subunit alpha, CTD kinase subunit 1, Latrunculin sensitive kinase 1
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
KEGG Pathway
spo:SPAC2F3.15
EC number
2.7.11.23: Protein-serine/threonine kinases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-239 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

416-440 (Activation loop from InterPro)

Target domain

277-561 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for O14098

Entry ID Method Resolution Chain Position Source
AF-O14098-F1 Predicted AlphaFoldDB

9 variants for O14098

Variant ID(s) Position Change Description Diseaes Association Provenance
I_3954615_A_G 49 K>E No Jeffares_SNPs
I_3954651_G_A 61 A>T No Jeffares_SNPs
I_3954725_T_G 85 H>Q No Jeffares_SNPs
I_3954754_G_A 95 R>K No Jeffares_SNPs
I_3955135_A_C 222 H>P No Jeffares_SNPs
I_3955859_C_T 426 S>F No Jeffares_SNPs
I_3956097_T_A 505 N>K No Jeffares_SNPs
I_3956200_A_C 540 K>Q No Jeffares_SNPs
I_3956287_G_A 569 E>K No Jeffares_SNPs

No associated diseases with O14098

3 regional properties for O14098

Type Name Position InterPro Accession
domain Protein kinase domain 277 - 561 IPR000719
active_site Serine/threonine-protein kinase, active site 395 - 407 IPR008271
binding_site Protein kinase, ATP binding site 283 - 306 IPR017441

Functions

Description
EC Number 2.7.11.23 Protein-serine/threonine kinases
Subcellular Localization
  • Nucleus, nucleolus
  • Localized to nucleus during all cell cycle phases
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

7 GO annotations of cellular component

Name Definition
chromatin The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome.
CTDK-1 complex A positive transcription elongation factor complex that comprises the CDK kinase CTK1 (in budding yeast), Lsk1 (in fission yeast) (corresponding to the Panther PTHR24056:SF39 family), a cyclin and an additional gamma subunit (corresponding to the InterPRO entry IPR024638).
cyclin-dependent protein kinase holoenzyme complex Cyclin-dependent protein kinases (CDKs) are enzyme complexes that contain a kinase catalytic subunit associated with a regulatory cyclin partner.
cyclin/CDK positive transcription elongation factor complex A transcription elongation factor complex that facilitates the transition from abortive to productive elongation by phosphorylating the CTD domain of the large subunit of DNA-directed RNA polymerase II, holoenzyme. Contains a cyclin and a cyclin-dependent protein kinase catalytic subunit.
nuclear cyclin-dependent protein kinase holoenzyme complex Cyclin-dependent protein kinase (CDK) complex found in the nucleus.
nucleolus A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
cyclin binding Binding to cyclins, proteins whose levels in a cell varies markedly during the cell cycle, rising steadily until mitosis, then falling abruptly to zero. As cyclins reach a threshold level, they are thought to drive cells into G2 phase and thus to mitosis.
cyclin-dependent protein serine/threonine kinase activity Cyclin-dependent catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
RNA polymerase II CTD heptapeptide repeat kinase activity Catalysis of the reaction: ATP + RNA polymerase II large subunit CTD heptapeptide repeat (YSPTSPS) = ADP + H+ + phosphorylated RNA polymerase II.

7 GO annotations of biological process

Name Definition
mRNA processing Any process involved in the conversion of a primary mRNA transcript into one or more mature mRNA(s) prior to translation into polypeptide.
phosphorylation of RNA polymerase II C-terminal domain The process of introducing a phosphate group on to an amino acid residue in the C-terminal domain of RNA polymerase II. Typically, this occurs during the transcription cycle and results in production of an RNA polymerase II enzyme where the carboxy-terminal domain (CTD) of the largest subunit is extensively phosphorylated, often referred to as hyperphosphorylated or the II(0) form. Specific types of phosphorylation within the CTD are usually associated with specific regions of genes, though there are exceptions. The phosphorylation state regulates the association of specific complexes such as the capping enzyme or 3'-RNA processing machinery to the elongating RNA polymerase complex.
positive regulation of transcription by RNA polymerase II Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter.
positive regulation of transcription elongation by RNA polymerase II Any process that activates or increases the frequency, rate or extent of transcription elongation, the extension of an RNA molecule after transcription initiation and promoter clearance by the addition of ribonucleotides, catalyzed by RNA polymerase II.
protein phosphorylation The process of introducing a phosphate group on to a protein.
signaling The entirety of a process in which information is transmitted within a biological system. This process begins with an active signal and ends when a cellular response has been triggered.
transcription elongation by RNA polymerase II promoter The extension of an RNA molecule after transcription initiation and promoter clearance at an RNA polymerase II promoter by the addition of ribonucleotides catalyzed by RNA polymerase II.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MSYSKSTIYR RQGTEPNSHF RRTVEEKSQL SGTNEESLGG HTLSSNAFKN NSSSISPSSS
70 80 90 100 110 120
AKDPREQRKR TFPLNDTHSS RARQHERPFR SRKSRRRKGK KAFSPRPGSP PSPSFYRSGS
130 140 150 160 170 180
QKRARNLTTK DYFAKRSESS SSASVSPISP SANRNDSKRQ ASSFRRSPPS SVHMKPSAFN
190 200 210 220 230 240
GRKVSRRPSS SPPPIPSIPH ETTSSDTQKK SSVSSGFPEN KHGKFHFHIP NERRSRFDQP
250 260 270 280 290 300
PSKRMALTST ARESVPAPLP SPPSGPIYTY TYPKPAYEKI DQIGEGTYGK VYKAINTVTG
310 320 330 340 350 360
DLVALKRIRL EQEKDGFPIT TVREVKILQR LRHKNIVRLL EIMVEKSSVY MVFEYMDHDL
370 380 390 400 410 420
TGVLLNSQLH FTPGNIKHLS KQIFEALAYL HHRGVLHRDI KGSNILLNNN GDLKFADFGL
430 440 450 460 470 480
ARFNTSSKSA NYTNRVITLW FRPPELLLGE TAYDTAVDIW SAGCIVMELF TGKPFFQGRD
490 500 510 520 530 540
EISQLEVIYD MMGTPDVHSW PEVKNLPWYE LLKPVEEKKS RFVETFKEIL SPAAIDLCQK
550 560 570 580 590
LLALNPFCRP SAHETLMHEY FTSESPPPEP AVILKNMQGS WHEWESKKRK SKR