O06582

PR
Gene name
prpD (Rv1130, RVBD_1130, P425_01179)
Protein name
2-methylcitrate dehydratase
Names
2-MC dehydratase, Aconitate hydratase, ACN, Aconitase
Species
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
KEGG Pathway
mtv:RVBD_1130
EC number
4.2.1.3: Hydro-lyases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

264-394 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for O06582

Entry ID Method Resolution Chain Position Source
AF-O06582-F1 Predicted AlphaFoldDB

No variants for O06582

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for O06582

No associated diseases with O06582

2 regional properties for O06582

Type Name Position InterPro Accession
domain MmgE/PrpD, N-terminal 45 - 286 IPR045336
domain MmgE/PrpD, C-terminal 309 - 495 IPR045337

Functions

Description
EC Number 4.2.1.3 Hydro-lyases
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
peptidoglycan-based cell wall A protective structure outside the cytoplasmic membrane composed of peptidoglycan (also known as murein), a molecule made up of a glycan (sugar) backbone of repetitively alternating N-acetylglucosamine and N-acetylmuramic acid with short, attached, cross-linked peptide chains containing unusual amino acids. An example of this component is found in Escherichia coli.

3 GO annotations of molecular function

Name Definition
2-methylcitrate dehydratase activity Catalysis of the reaction: (2S,3S)-2-methylcitrate = cis-2-methylaconitate + H(2)O.
aconitate hydratase activity Catalysis of the reaction: citrate = isocitrate. The reaction occurs in two steps: (1) citrate = cis-aconitate + H2O, (2) cis-aconitate + H2O = isocitrate. This reaction is the interconversion of citrate and isocitrate via the labile, enzyme-bound intermediate cis-aconitate. Water is removed from one part of the citrate molecule and added back to a different atom to form isocitrate.
citrate dehydratase activity Catalysis of the reaction: citrate = cis-aconitate + H2O.

4 GO annotations of biological process

Name Definition
cholesterol metabolic process The chemical reactions and pathways involving cholesterol, cholest-5-en-3 beta-ol, the principal sterol of vertebrates and the precursor of many steroids, including bile acids and steroid hormones. It is a component of the plasma membrane lipid bilayer and of plasma lipoproteins and can be found in all animal tissues.
propionate catabolic process, 2-methylcitrate cycle The chemical reactions and pathways resulting in the breakdown of propionate that occurs in the 2-methylcitrate cycle.
response to acidic pH Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a pH stimulus with pH < 7. pH is a measure of the acidity or basicity of an aqueous solution.
tricarboxylic acid cycle A nearly universal metabolic pathway in which the acetyl group of acetyl coenzyme A is effectively oxidized to two CO2 and four pairs of electrons are transferred to coenzymes. The acetyl group combines with oxaloacetate to form citrate, which undergoes successive transformations to isocitrate, 2-oxoglutarate, succinyl-CoA, succinate, fumarate, malate, and oxaloacetate again, thus completing the cycle. In eukaryotes the tricarboxylic acid is confined to the mitochondria. See also glyoxylate cycle.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MPDQDTKVRF FRVFCWCPVL RMVRIMLMHA VRAWRSADDF PCTEHMAYKI AQVAADPVDV
70 80 90 100 110 120
DPEVADMVCN RIIDNAAVSA ASMVRRPVTV ARHQALAHPV RHGAKVFGVE GSYSADWAAW
130 140 150 160 170 180
ANGVAARELD FHDTFLAADY SHPADNIPPL VAVAQQLGVC GAELIRGLVT AYEIHIDLTR
190 200 210 220 230 240
GICLHEHKID HVAHLGPAVA AGIGTMLRLD QETIYHAIGQ ALHLTTSTRQ SRKGAISSWK
250 260 270 280 290 300
AFAPAHAGKV GIEAVDRAMR GEGSPAPIWE GEDGVIAWLL AGPEHTYRVP LPAPGEPKRA
310 320 330 340 350 360
ILDSYTKQHS AEYQSQAPID LACRLRERIG DLDQIASIVL HTSHHTHVVI GTGSGDPQKF
370 380 390 400 410 420
DPDASRETLD HSLPYIFAVA LQDGCWHHER SYAPERARRS DTVALWHKIS TVEDPEWTRR
430 440 450 460 470 480
YHCADPAKKA FGARAEVTLH SGEVIVDELA VADAHPLGTR PFERKQYVEK FTELADGVVE
490 500 510 520
PVEQQRFLAV VESLADLESG AVGGLNVLVD PRVLDKAPVI PPGIFR