D0ZPH9

SS
Gene name
sspH2 (STM14_2769)
Protein name
E3 ubiquitin-protein ligase SspH2
Names
RING-type E3 ubiquitin transferase SspH2, Salmonella secreted protein H2, Secreted effector protein sspH2
Species
Salmonella typhimurium (strain 14028s / SGSC 2262)
KEGG Pathway
seo:STM14_2769
EC number
2.3.2.27: Aminoacyltransferases
Protein Class

Descriptions

SspH2, consisting of an LRR domain and a NEL domain, is an E3 ubiquitin ligase with unique folding. The NEL domain has a catalytic activity that is inhibited by the LRR domain by sequestering the catalytic cysteine ??residue contained in the NEL domain.

Autoinhibitory domains (AIDs)

223-466 (LRR domain) SS

Target domain

502-714 (Novel E3 ligase, NEL domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for D0ZPH9

Entry ID Method Resolution Chain Position Source
AF-D0ZPH9-F1 Predicted AlphaFoldDB

No variants for D0ZPH9

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for D0ZPH9

No associated diseases with D0ZPH9

10 regional properties for D0ZPH9

Type Name Position InterPro Accession
repeat Leucine-rich repeat 243 - 264 IPR001611-1
repeat Leucine-rich repeat 303 - 324 IPR001611-2
repeat Leucine-rich repeat 343 - 364 IPR001611-3
repeat Leucine-rich repeat 383 - 404 IPR001611-4
repeat Leucine-rich repeat, typical subtype 242 - 267 IPR003591-1
repeat Leucine-rich repeat, typical subtype 302 - 327 IPR003591-2
repeat Leucine-rich repeat, typical subtype 340 - 367 IPR003591-3
repeat Leucine-rich repeat, typical subtype 380 - 403 IPR003591-4
repeat Leucine-rich repeat, typical subtype 420 - 444 IPR003591-5
domain Novel E3 ligase domain 502 - 714 IPR029487

Functions

Description
EC Number 2.3.2.27 Aminoacyltransferases
Subcellular Localization
  • Secreted
  • Host cytoplasm
  • Host apical cell membrane; Peripheral membrane protein; Cytoplasmic side
  • Secreted via type III secretion system 2 (SPI-2 T3SS), and delivered into the host cytoplasm
  • Localizes at the periphery of the host cell, specifically in areas of actin polymerization
  • Localizes to the apical membrane of polarized epithelial cells
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
extracellular region The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
host cell cytoplasm The cytoplasm of a host cell.
host cell plasma membrane The plasma membrane surrounding a host cell.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.

1 GO annotations of molecular function

Name Definition
ubiquitin-protein transferase activity Catalysis of the transfer of ubiquitin from one protein to another via the reaction X-Ub + Y --> Y-Ub + X, where both X-Ub and Y-Ub are covalent linkages.

2 GO annotations of biological process

Name Definition
protein secretion by the type III secretion system The process in which proteins are transferred into the extracellular milieu or directly into host cells by the bacterial type III secretion system; secretion occurs in a continuous process without the distinct presence of periplasmic intermediates and does not involve proteolytic processing of secreted proteins.
protein ubiquitination The process in which one or more ubiquitin groups are added to a protein.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MPFHIGSGCL PATISNRRIY RIAWSDTPPE MSSWEKMKEF FCSTHQTEAL ECIWTICHPP
70 80 90 100 110 120
AGTTREDVIN RFELLRTLAY AGWEESIHSG QHGENYFCIL DEDSQEILSV TLDDAGNYTV
130 140 150 160 170 180
NCQGYSETHR LTLDTAQGEE GTGHAEGASG TFRTSFLPAT TAPQTPAEYD AVWSAWRRAA
190 200 210 220 230 240
PAEESRGRAA VVQKMRACLN NGNAVLNVGE SGLTTLPDCL PAHITTLVIP DNNLTSLPAL
250 260 270 280 290 300
PPELRTLEVS GNQLTSLPVL PPGLLELSIF SNPLTHLPAL PSGLCKLWIF GNQLTSLPVL
310 320 330 340 350 360
PPGLQELSVS DNQLASLPAL PSELCKLWAY NNQLTSLPML PSGLQELSVS DNQLASLPTL
370 380 390 400 410 420
PSELYKLWAY NNRLTSLPAL PSGLKELIVS GNRLTSLPVL PSELKELMVS GNRLTSLPML
430 440 450 460 470 480
PSGLLSLSVY RNQLTRLPES LIHLSSETTV NLEGNPLSER TLQALREITS APGYSGPIIR
490 500 510 520 530 540
FDMAGASAPR ETRALHLAAA DWLVPAREGE PAPADRWHMF GQEDNADAFS LFLDRLSETE
550 560 570 580 590 600
NFIKDAGFKA QISSWLAQLA EDEALRANTF AMATEATSSC EDRVTFFLHQ MKNVQLVHNA
610 620 630 640 650 660
EKGQYDNDLA ALVATGREMF RLGKLEQIAR EKVRTLALVD EIEVWLAYQN KLKKSLGLTS
670 680 690 700 710 720
VTSEMRFFDV SGVTVTDLQD AELQVKAAEK SEFREWILQW GPLHRVLERK APERVNALRE
730 740 750 760 770 780
KQISDYEETY RMLSDTELRP SGLVGNTDAE RTIGARAMES AKKTFLDGLR PLVEEMLGSY
LNVQWRRN