B5Y235

PR
Gene name
ileS
Protein name
Isoleucine--tRNA ligase
Names
Isoleucyl-tRNA synthetase, IleRS
Species
Klebsiella pneumoniae (strain 342)
KEGG Pathway
kpe:KPK_4735
EC number
6.1.1.5: Ligases forming aminoacyl-tRNA and related compounds
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-50 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for B5Y235

Entry ID Method Resolution Chain Position Source
AF-B5Y235-F1 Predicted AlphaFoldDB

No variants for B5Y235

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for B5Y235

No associated diseases with B5Y235

5 regional properties for B5Y235

Type Name Position InterPro Accession
conserved_site Aminoacyl-tRNA synthetase, class I, conserved site 58 - 69 IPR001412
domain Aminoacyl-tRNA synthetase, class Ia 28 - 640 IPR002300
domain Zinc finger, FPG/IleRS-type 898 - 926 IPR010663
domain Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding 685 - 840 IPR013155
domain Isoleucyl tRNA synthetase type 1, anticodon-binding domain 640 - 820 IPR033708

Functions

Description
EC Number 6.1.1.5 Ligases forming aminoacyl-tRNA and related compounds
Subcellular Localization
  • Cytoplasm
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

1 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

5 GO annotations of molecular function

Name Definition
aminoacyl-tRNA editing activity The hydrolysis of an incorrectly aminoacylated tRNA.
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
isoleucine-tRNA ligase activity Catalysis of the reaction: L-isoleucine + ATP + tRNA(Ile) = L-isoleucyl-tRNA(Ile) + AMP + diphosphate + 2 H(+).
tRNA binding Binding to a transfer RNA.
zinc ion binding Binding to a zinc ion (Zn).

1 GO annotations of biological process

Name Definition
isoleucyl-tRNA aminoacylation The process of coupling isoleucine to isoleucyl-tRNA, catalyzed by isoleucyl-tRNA synthetase. The isoleucyl-tRNA synthetase is a class-I synthetase. The activated amino acid is transferred to the 2'-OH group of a isoleucine-accetping tRNA. The 2'-O-aminoacyl-tRNA will ultimately migrate to the 3' position via transesterification.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MSDYKSTLNL PETGFPMRGD LAKREPGMLA RWTDDDLYGI IRAAKKGKKT FILHDGPPYA
70 80 90 100 110 120
NGSIHIGHSV NKILKDIIVK SKGLTGYDSP YVPGWDCHGL PIELKVEQQY GKPGEKFTAA
130 140 150 160 170 180
EFRSKCREYA AEQIDGQRKD FIRLGVLGDW SRPYLTMDFK TEANIIRALG KIIGNGHLHK
190 200 210 220 230 240
GAKPVHWCVD CRSALAEAEV EYYDKTSPSI DVAFHAVDKA AVLAKFGVAD VNGPVSLVIW
250 260 270 280 290 300
TTTPWTLPAN RAISLSPEFD YALVQVDGQA LILAKDLVES VMKRVGATDY TILAAVQGSE
310 320 330 340 350 360
LELMRFKHPF LDFDVPAILG DHVTLDAGTG AVHTAGGHGP DDYTISQKYG LEIANPVGPD
370 380 390 400 410 420
GAYLPGTYPS LDGINVFKAN DIIVEMLRDS GALLHVEKMQ HSYPCCWRHK SPIIFRATPQ
430 440 450 460 470 480
WFVSMDQKGL RAQSLKEIKG VQWIPDWGQA RIESMVANRP DWCISRQRTW GVPMSLFVHK
490 500 510 520 530 540
ETHELHPRTL ELMEEVAKRV EVDGIQAWWD LDSRDILGDD ADSYEKVPDT LDVWFDSGST
550 560 570 580 590 600
HSSVVDVRPE FAGHAADMYL EGSDQHRGWF MSSLMISTAM KGKAPYRQVL THGFTVDGQG
610 620 630 640 650 660
RKMSKSIGNT VSPQDVMNKL GADILRLWVA STDYTGEMAV SDEILKRAAD SYRRIRNTAR
670 680 690 700 710 720
FLLANLNGFD PAKDMVKPEE MVVLDRWAVG CAQAAQEDIL KAYESYDFHE VVQRLMRFCS
730 740 750 760 770 780
IEMGSFYLDI IKDRQYTAKA DSVARRSCQT ALFHIVEALV RWMAPIMSFT ADEIWGYLPG
790 800 810 820 830 840
DREKYVFTGE WYKGLFGLAD DEAMNDGFWD ELLKVRGEVN KVIEQARADK KVGGSLEAAV
850 860 870 880 890 900
TLYADADLAA KLNALGDELR FVLLTSGANV ADYAQAPADA WQSDLLKGLK VVLSKAEGEK
910 920 930
CPRCWHYTSD VGKVAEHAEI CGRCVSNVAG NGEQRKFA