B5DFI8

SS
Gene name
Ube2s
Protein name
Ubiquitin-conjugating enzyme E2 S
Names
EC 2.3.2.23 , E2 ubiquitin-conjugating enzyme S , Ubiquitin carrier protein S , Ubiquitin-protein ligase S
Species
Bos taurus (Bovine)
KEGG Pathway
rno:292588
EC number
Protein Class

Descriptions

NFAT1 plays a role in the inducible expression of cytokine genes in T-cells. NFAT transcription factors are highly phosphorylated proteins that are regulated by the calcium-dependent phosphatase calcineurin. NFAT1 is phosphorylated on fourteen conserved phosphoserine residues in its regulatory domain (100-400), thirteen of which are dephosphorylated upon stimulation. Dephosphorylation of all thirteen residues is required to mask a nuclear export signal (NES), causes full exposure of a nuclear localization signal (NLS), and promotes transcriptional activity. Mutations in the serine-rich region (SRR-1, specifically 170-183) enhances the localization of NFAT1 to the nucleus, while the full-length NFAT1 does not move into the nucleus. This represents that the SRR-1 region inhibits the translocation of NFAT1 to the nucleus. In addition, SRR-1 region also regulates the active conformation of NFAT1.

Autoinhibitory domains (AIDs)

198-223 (C-helix) SS

Target domain

1-156 (UBC domain)

Relief mechanism

PTM

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for B5DFI8

Entry ID Method Resolution Chain Position Source
AF-B5DFI8-F1 Predicted AlphaFoldDB

1 variants for B5DFI8

Variant ID(s) Position Change Description Diseaes Association Provenance
rs8157783 170 R>Q No EVA

No associated diseases with B5DFI8

1 regional properties for B5DFI8

Type Name Position InterPro Accession
domain Small GTP-binding protein domain 24 - 167 IPR005225

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm
  • Nucleus
  • Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation
  • Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals
  • Translocation to the nucleus is increased in the presence of calcium in pre-osteoblasts (By similarity)
  • The subcellular localization of NFATC plays a key role in the regulation of gene transcription
  • Nuclear translocation OF NFATC1 is enhanced in the presence of TNFSF11
  • Nuclear translocation is decreased in the presence of FBN1 which can bind and sequester TNFSF11
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
anaphase-promoting complex A ubiquitin ligase complex that degrades mitotic cyclins and anaphase inhibitory protein, thereby triggering sister chromatid separation and exit from mitosis. Substrate recognition by APC occurs through degradation signals, the most common of which is termed the Dbox degradation motif, originally discovered in cyclin B.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
endoplasmic reticulum The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisternae. The ER takes two forms, rough (or granular), with ribosomes adhering to the outer surface, and smooth (with no ribosomes attached).
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

4 GO annotations of molecular function

Name Definition
anaphase-promoting complex binding Binding to an anaphase-promoting complex. A ubiquitin ligase complex that degrades mitotic cyclins and anaphase inhibitory protein, thereby triggering sister chromatid separation and exit from mitosis.
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ubiquitin conjugating enzyme activity Isoenergetic transfer of ubiquitin from one protein to another via the reaction X-ubiquitin + Y -> Y-ubiquitin + X, where both the X-ubiquitin and Y-ubiquitin linkages are thioester bonds between the C-terminal glycine of ubiquitin and a sulfhydryl side group of a cysteine residue.
ubiquitin-protein transferase activity Catalysis of the transfer of ubiquitin from one protein to another via the reaction X-Ub + Y --> Y-Ub + X, where both X-Ub and Y-Ub are covalent linkages.

12 GO annotations of biological process

Name Definition
anaphase-promoting complex-dependent catabolic process The chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of ubiquitin, with ubiquitin-protein ligation catalyzed by the anaphase-promoting complex, and mediated by the proteasome.
cell division The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.
exit from mitosis The cell cycle transition where a cell leaves M phase and enters a new G1 phase. M phase is the part of the mitotic cell cycle during which mitosis and cytokinesis take place.
free ubiquitin chain polymerization The process of creating free ubiquitin chains, compounds composed of a large number of ubiquitin monomers. These chains are not conjugated to a protein.
positive regulation of ubiquitin protein ligase activity Any process that activates or increases the frequency, rate or extent of ubiquitin protein ligase activity.
protein K11-linked ubiquitination A protein ubiquitination process in which ubiquitin monomers are attached to a protein, and then ubiquitin polymers are formed by linkages between lysine residues at position 11 of the ubiquitin monomers. K11-linked polyubiquitination targets the substrate protein for degradation. The anaphase-promoting complex promotes the degradation of mitotic regulators by assembling K11-linked polyubiquitin chains.
protein K27-linked ubiquitination A protein ubiquitination process in which a polymer of ubiquitin, formed by linkages between lysine residues at position 27 of the ubiquitin monomers, is added to a protein.
protein K29-linked ubiquitination A protein ubiquitination process in which a polymer of ubiquitin, formed by linkages between lysine residues at position 29 of the ubiquitin monomers, is added to a protein. K29-linked ubiquitination targets the substrate protein for degradation.
protein K6-linked ubiquitination A protein ubiquitination process in which a polymer of ubiquitin, formed by linkages between lysine residues at position 6 of the ubiquitin monomers, is added to a protein. K6-linked ubiquitination is involved in DNA repair.
protein K63-linked ubiquitination A protein ubiquitination process in which a polymer of ubiquitin, formed by linkages between lysine residues at position 63 of the ubiquitin monomers, is added to a protein. K63-linked ubiquitination does not target the substrate protein for degradation, but is involved in several pathways, notably as a signal to promote error-free DNA postreplication repair.
protein polyubiquitination Addition of multiple ubiquitin groups to a protein, forming a ubiquitin chain.
ubiquitin-dependent protein catabolic process The chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of a ubiquitin group, or multiple ubiquitin groups, to the protein.

8 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q1RML1 UBE2S Ubiquitin-conjugating enzyme E2 S Bos taurus (Bovine) SS
Q9VX25 CG8188 Ubiquitin-conjugating enzyme E2 S Drosophila melanogaster (Fruit fly) SS
Q16763 UBE2S Ubiquitin-conjugating enzyme E2 S Homo sapiens (Human) EV
Q921J4 Ube2s Ubiquitin-conjugating enzyme E2 S Mus musculus (Mouse) SS
Q5FVH4 Aktip AKT-interacting protein Rattus norvegicus (Rat) PR
Q9FF66 UBC22 Ubiquitin-conjugating enzyme E2 22 Arabidopsis thaliana (Mouse-ear cress) SS
Q28F89 ube2s Ubiquitin-conjugating enzyme E2 S Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SS
Q4V908 ube2s Ubiquitin-conjugating enzyme E2 S Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MTGLEEDQEF DFDFLFEFNQ SDEGAVAAPA EHYGYAAPGL GAGLPLSAAH PSLPAPCHDL
70 80 90 100 110 120
QSSAAGVSAV GYGGTVDSGP SGYFLSSGGI RPNGAPALES PRIEITSYLG LHHNSSQFLH
130 140 150 160 170 180
EVDVEDVLPK RSPSTATLNL PSLEAYRDPS CLSPASSLSS RSCNSEASSY ESSFSYPYAS
190 200 210 220 230 240
PQTSPWQSPC VSPKTTDPEE GFARGLGACG LLGSPRHSPS TSPRTSVTEE SWLGARTSRP
250 260 270 280 290 300
SSPCNKRKYG LNGRQLSCSP HPSPTPSPQG SPRVSVTDDT WLGNTTQYTS SAIVAAINAL
310 320 330 340 350 360
STDSSLDLGD GVPVKARKTT LDHSPAVALK VEPAGEDLGT TPPTPDFQPE EFAAFQHIRK
370 380 390 400 410 420
GAFCDQYLSV PQHPYPWARP RSPASYTSPS LPALDWQLPS HSGPYELRIE VQPKSHHRAH
430 440 450 460 470 480
YETEGSRGAV KASAGGHPSV QLHGYVESEP LTLQLFIGTA DDRLLRPHAF YQVHRITGKT
490 500 510 520 530 540
VSTASHEAVV CSTKVLEIPL LPENNMRATI DCAGILKLRN SDIELRKGET DIGRKNTRVR
550 560 570 580 590 600
LVFRVHIPQP NGRTLSLQVA SNPIECSQRS AQELPLVEKQ SAASGPVLGG KRMVLSGHNF
610 620 630 640 650 660
LQDSKVIFVE KAPDGHHIWE MEAKTEGDLC KPNSLVVEIP PFRNQRITSP VQVNFYVCNG
670 680 690 700 710 720
KRKRSQYQHF TYLPANVPII KTEPSDDYEP ALTCGPMSQG LSPLPKPCYG QPLALPPDPG
730 740 750 760 770 780
ACLMPGFPPR PQGSASPELH DLSCAPYGSA TAGPGHSPLG LPRPVGGVLA SQEAPRPSGV
790 800
PPGPPQPPPP TLLQPQVSPT SSG